Yu Xiaomin, Fu Xujun, Yang Qinghua, Jin Hangxia, Zhu Longming, Yuan Fengjie
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Plants (Basel). 2021 Dec 23;11(1):28. doi: 10.3390/plants11010028.
Vegetable soybean is a type of value-added specialty soybean, served as a fresh vegetable or snack in China. Due to the difference from other types, it is important to understand the genetic structure and diversity of vegetable soybean for further utilization in breeding programs. The four vegetable cultivars, Taiwan-75, Zhexiandou No. 8, Zhexian No. 9 and Zhexian No. 10 are popular soybean varieties planted in Zhejiang province, and have large pods and intermediate maturity. The clustering showed a close relationship of these four cultivars in simple sequence repeat analysis. To reveal the genome variation of vegetable soybean, these four improved lines were analyzed by whole-genome re-sequencing. The average sequencing depth was 7X and the coverage ratio of each cultivar was at least more than 94%. Compared with the reference genome, a large number of single-nucleotide polymorphisms, insertion/deletions and structure variations were identified with different chromosome distributions. The average heterozygosity rate of the single-nucleotide polymorphisms was 11.99% of these four cultivars. According to the enrichment analysis, there were 23,371 genes identified with putative modifications, and a total of 282 genes were related to carbohydrate metabolic processes. These results provide useful information for genetic research and future breeding, which can facilitate the selection procedures in vegetable soybean breeding.
毛豆是一种增值型特色大豆，在中国作为新鲜蔬菜或小吃食用。由于与其他类型不同，了解毛豆的遗传结构和多样性对于在育种计划中的进一步利用很重要。台湾75、浙鲜豆8号、浙鲜9号和浙鲜10号这四个毛豆品种是浙江省种植的受欢迎的大豆品种，豆荚大且成熟期适中。聚类分析表明，在简单序列重复分析中这四个品种关系密切。为了揭示毛豆的基因组变异，对这四个改良品系进行了全基因组重测序分析。平均测序深度为7X，每个品种的覆盖比率至少超过94%。与参考基因组相比，鉴定出大量单核苷酸多态性、插入/缺失和结构变异，且具有不同的染色体分布。这四个品种单核苷酸多态性的平均杂合率为11.99%。根据富集分析，鉴定出23371个具有推定修饰的基因，共有282个基因与碳水化合物代谢过程相关。这些结果为遗传研究和未来育种提供了有用信息，有助于毛豆育种的选择过程。
