高静水压预处理对米糠蛋白水解物功能和结构特性的影响

Effects of High Hydrostatic Pressure Pretreatment on the Functional and Structural Properties of Rice Bran Protein Hydrolysates.

作者信息

Wang Shirang, Wang Tengyu, Sun Yue, Cui Yingju, Yu Guoping, Jiang Lianzhou

机构信息

College of Food Science, Northeast Agricultural University, Harbin 150030, China.

School of Grain Engineering, Heilongjiang Communications Polytechnic, Harbin 150025, China.

出版信息

Foods. 2021 Dec 23;11(1):29. doi: 10.3390/foods11010029.

Rice bran protein (RBP) hydrolysis was conducted after high hydrostatic pressure (HHP) pretreatment. The structural and functional properties of HHP-pretreated rice bran protein hydrolysates (RBPH) were investigated. HHP pretreatments were conducted at 100, 200, and 300 MPa; then, enzymatic hydrolysis at atmospheric pressure was performed using trypsin. An RBPH sample that had not been pretreated by HHP was used as a control. Free sulfhydryl (SH) content, SDS-PAGE profiles, high-performance size exclusion chromatography (HPSEC), Fourier transform infrared (FTIR) spectrum, scanning electron microscopy (SEM), intrinsic fluorescence spectrum, solubility, and emulsifying and foaming properties were evaluated. Changes in particle size and ζ-potential were monitored. Compared with the control, the results of solubility, the emulsifying activity index (EAI) and the emulsifying stability index (ESI) increased significantly ( < 0.05) at 200 MPa. The content of free SH increased significantly ( < 0.05) at 100 MPa. FTIR spectrum and fluorescence analysis confirmed the changes in the secondary and tertiary structures. The experimental results indicated that the structural and functional properties of HHP-pretreated RBPH improved.

在高静水压（HHP）预处理后进行米糠蛋白（RBP）水解。研究了经HHP预处理的米糠蛋白水解物（RBPH）的结构和功能特性。HHP预处理在100、200和300MPa下进行；然后，在常压下使用胰蛋白酶进行酶水解。将未经HHP预处理的RBPH样品用作对照。评估了游离巯基（SH）含量、SDS-PAGE图谱、高效尺寸排阻色谱（HPSEC）、傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）、内源荧光光谱、溶解度以及乳化和发泡性能。监测了粒径和ζ电位的变化。与对照相比，在200MPa下溶解度、乳化活性指数（EAI）和乳化稳定性指数（ESI）的结果显著增加（<0.05）。在100MPa下游离SH含量显著增加（<0.05）。FTIR光谱和荧光分析证实了二级和三级结构的变化。实验结果表明，经HHP预处理的RBPH的结构和功能特性得到了改善。