Silva Lúcia Daniel Machado da
Laboratory of Carnivores Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza 60714-903, Ceará, Brazil.
Animals (Basel). 2022 Jan 5;12(1):124. doi: 10.3390/ani12010124.
The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.
人们对繁育犬猫以及将它们用作其他犬科和猫科动物模型的兴趣日益增加，这就需要开展研究来改进繁殖技术。其中，睾丸冷冻保存尤为突出。睾丸冷冻保存能够维持生殖能力，并为具有商业价值或面临灭绝风险的多个物种建立种质库。此外，它还能在不同地区之间运输遗传物质。值得注意的是，这种生物技术是保存已死亡青春期前动物生育能力的唯一可能方式，因此在动物遗传物质的繁殖方面具有重要意义。睾丸中存在的精原细胞可以在体外培养，获得的精子可用于人工繁殖计划。尽管在使用睾丸碎片获取有活力和功能的生殖细胞方面已取得进展，但尚未得出可用于临床常规的方案。睾丸冷冻保存过程可以通过慢速冷冻、快速冷冻和玻璃化等技术进行。然而，用于犬猫物种的方案仍处于实验阶段。鉴于该主题的重要性，本综述的目的是勾勒出该主题的概况，介绍犬猫睾丸冷冻保存方面的主要研究成果。
