Department of Food Science, National Ilan University, Number 1, Section 1, Shen-Lung Road, Yilan City 260007, Taiwan.
Department of Biotechnology and Animal Science, National Ilan University, Number 1, Section 1, Shen-Lung Road, Yilan City 260007, Taiwan.
Molecules. 2021 Dec 24;27(1):109. doi: 10.3390/molecules27010109.
Solid-state fermentation may produce therapeutic compounds with higher biomass or better product characteristics than those generated by submerged fermentation. The objectives of this study were to analyze the antioxidant activities and biosafety of products obtained by white truffle () solid-state fermentation in media with different ratios of soybean and red adlay. High levels of antioxidant components and high antioxidant activities such as DPPH radical scavenging, ferrous ion chelation, and reducing power were measured in 20 mg/mL water and ethanol extracts of the white truffle fermentation products. When the solid-state fermentation medium contained soybean and red adlay in a 1:3 ratio (S1A3), the fermentation product had more uniform antioxidant compositions and activities by principal component analysis (PCA). In addition, a 200 ppm water extract of the mycelial fermentation product was able to protect zebrafish embryos from oxidative stress induced by 5 mM hydrogen peroxide. Sprague-Dawley rats were fed the mycelial fermentation product for 90 consecutive days, revealing a no-observed-adverse-effect level (NOAEL) of 3000 mg/kg BW/day. Therefore, mycelial products obtained by white truffle solid-state fermentation can be used instead of expensive fruiting bodies as a good source of antioxidant ingredients.
固态发酵可能会产生比液体发酵更高生物量或更好产品特性的治疗化合物。本研究的目的是分析不同大豆和红米比例的培养基中白松露（）固态发酵产物的抗氧化活性和生物安全性。在白松露发酵产物的 20mg/mL 水和乙醇提取物中，检测到了高水平的抗氧化成分和高抗氧化活性，如 DPPH 自由基清除、亚铁离子螯合和还原能力。当固态发酵培养基中大豆和红米的比例为 1:3（S1A3）时，通过主成分分析（PCA），发酵产物具有更均匀的抗氧化成分和活性。此外，菌丝体发酵产物的 200ppm 水提取物能够保护斑马鱼胚胎免受 5mM 过氧化氢诱导的氧化应激。连续 90 天给 Sprague-Dawley 大鼠喂食菌丝体发酵产物，未观察到不良效应水平（NOAEL）为 3000mg/kgBW/天。因此，白松露固态发酵获得的菌丝体产物可以代替昂贵的子实体，成为抗氧化成分的良好来源。
