Division of Fundamental Agriscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-8589, Japan.
Graduate School of Global Food Resources, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo 060-0809, Japan.
Molecules. 2021 Dec 28;27(1):161. doi: 10.3390/molecules27010161.
Abscisic acid (ABA, ) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of . As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds.
脱落酸（ABA）是一种植物激素，调节种子发育和应激反应等各种植物生理过程。ABA 信号系统已经阐明；ABA 与 PYL 蛋白结合触发 ABA 信号。我们之前报道了一种使用带有炔基和氨基的生物素接头、蛋白质交联剂和带有叠氮基团的生物活性小分子（叠氮探针）分离与生物活性小分子靶标的蛋白质的新方法。该方法用于鉴定的未知 ABA 结合蛋白。结果，鉴定到硫氧还蛋白 AtTrxh3 为 ABA 结合蛋白。我们开发的方法可用于鉴定生物活性化合物的结合蛋白。
