巴拉圭马匹中动物利什曼原虫的首次分子调查。

First molecular survey of animal trypanosomes in Paraguayan horses.

机构信息

Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan.

Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido, Japan; Universidad Nacional de Canendiyu, Salto del Guaira, Paraguay.

出版信息

Vet Parasitol Reg Stud Reports. 2022 Jan;27:100664. doi: 10.1016/j.vprsr.2021.100664. Epub 2021 Nov 19.

DOI:10.1016/j.vprsr.2021.100664

PMID:35012722

Abstract

Despite the epidemic situation of animal trypanosomosis caused by Trypanosoma evansi, Trypanosoma equiperdum and Trypanosoma vivax in South American countries, there are no reports for the prevalence of animal trypanosomes in Paraguay. In this study, 408 blood samples were obtained from apparently healthy horses from sixteen departments of Paraguay, for routine medical check-up from August to September 2019, and a polymerase chain reaction (PCR)-based cross-sectional study was carried out to identify trypanosome prevalence. The prevalence of Trypanozoon (T. evansi and T. equiperdum) and T. vivax was 7.11% (29/408) and 26.23% (107/408), respectively. Mixed infections were detected in 4.90% (20/408) of the samples. Some of the selected trypanosome positive samples were confirmed as T. vivax and T. evansi Type A by sequence analysis of the internal transcribe spacer region and RoTat1.2 variant surface glycoprotein gene, respectively. In conclusion, we found higher prevalence of T. vivax than Trypanozoon in Paraguayan horses. However, the genotypic variation should be verified in further studies.

摘要

尽管南美国家存在由伊氏锥虫、马媾疫锥虫和布氏锥虫引起的动物锥虫病疫情，但巴拉圭尚无动物锥虫病流行的报告。本研究于 2019 年 8 月至 9 月，从巴拉圭 16 个省的 408 匹貌似健康的马中采集血液样本，用于常规医疗检查，并开展基于聚合酶链反应（PCR）的横断面研究，以确定锥虫病的流行情况。结果显示，伊氏锥虫和马媾疫锥虫的流行率分别为 7.11%（29/408）和 26.23%（107/408），混合感染率为 4.90%（20/408）。对部分阳性样本的序列分析表明，内部转录间隔区和 RoTat1.2 变异表面糖蛋白基因分别证实为布氏锥虫和伊氏锥虫 A 型。综上，本研究发现巴拉圭马中的布氏锥虫病流行率高于伊氏锥虫病，但进一步研究仍需验证其基因型变异。