Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-Hangi cho, Sakyo-ku, Kyoto, 606-0823, Japan.
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, 615-8510, Japan.
Nat Commun. 2022 Jan 10;13(1):96. doi: 10.1038/s41467-021-27707-5.
Ambient temperature significantly affects developmental timing in animals. The temperature sensitivity of embryogenesis is generally believed to be a consequence of the thermal dependency of cellular metabolism. However, the adaptive molecular mechanisms that respond to variations in temperature remain unclear. Here, we report species-specific thermal sensitivity of Notch signaling in the developing amniote brain. Transient hypothermic conditions increase canonical Notch activity and reduce neurogenesis in chick neural progenitors. Increased biosynthesis of phosphatidylethanolamine, a major glycerophospholipid components of the plasma membrane, mediates hypothermia-induced Notch activation. Furthermore, the species-specific thermal dependency of Notch signaling is associated with developmental robustness to altered Notch signaling. Our results reveal unique regulatory mechanisms for temperature-dependent neurogenic potentials that underlie developmental and evolutionary adaptations to a range of ambient temperatures in amniotes.
环境温度会显著影响动物的发育时间。胚胎发生的温度敏感性通常被认为是细胞代谢对温度依赖性的结果。然而，适应温度变化的分子机制尚不清楚。在这里，我们报告了在羊膜动物大脑发育过程中 Notch 信号的种特异性热敏感性。短暂的低温条件会增加经典 Notch 活性并减少鸡神经祖细胞的神经发生。磷脂酰乙醇胺（一种质膜的主要甘油磷脂成分）生物合成的增加介导了低温诱导的 Notch 激活。此外，Notch 信号的种特异性热依赖性与 Notch 信号改变时的发育稳健性有关。我们的研究结果揭示了温度依赖性神经发生潜力的独特调节机制，这些机制是羊膜动物对不同环境温度的发育和进化适应的基础。
