Austin Fertility & Reproductive Medicine/Westlake IVF, 300 Beardsley Lane, Bldg B, Suite 200, Austin, TX, 78746, USA.
Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, SC, 29605, USA.
J Assist Reprod Genet. 2022 Feb;39(2):389-394. doi: 10.1007/s10815-022-02393-4. Epub 2022 Jan 11.
The aim of this study was to determine if pregnancy-associated plasma protein-A (PAPP-A), typically measured in maternal serum and a potential predictor of adverse maternal and fetal outcomes such as spontaneous miscarriage, pre-eclampsia, and stillbirth, is expressed in blastocoel fluid-conditioned media (BFCM) at the embryonic blastocyst stage.
This is an in vitro study.
BFCM samples from trophectoderm-tested euploid blastocysts (n = 80) from in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) patients were analyzed for PAPP-A mRNA. BFCM was obtained from blastocyst stage embryos in 20 uL drops. Blastocysts underwent trophectoderm biopsy for preimplantation genetic testing for aneuploidy prior to blastocyst vitrification and BFCM collection for snap freezing. cfDNA was synthesized using BFCM collected from 80 individual euploid blastocysts. Next, real-time qPCR was performed to detect expression of PAPP-A with GAPDH for normalization of expression in each sample.
PAPP-A mRNA was detected in 45 of 80 BFCM samples (56.3%), with varying levels of expression across samples.
Our study demonstrates the expression of PAPP-A in BFCM. To our knowledge, this is the first study to report detection of PAPP-A mRNA in BFCM. Further studies are required and underway to investigate a greater number of BFCM samples as well as the possible correlation of PAPP-A expression with pregnancy outcomes of transferred euploid blastocysts. If found to predict IVF and obstetric outcomes, PAPP-A may provide additional information along with embryonic euploidy for the selection of the optimal blastocyst for embryo transfer.
本研究旨在确定妊娠相关血浆蛋白 A（PAPP-A）是否在囊胚腔液条件培养基（BFCM）中表达，该蛋白通常在母体血清中测量，是自发性流产、子痫前期和死胎等不良母婴结局的潜在预测指标。
这是一项体外研究。
分析了来自体外受精/胞浆内精子注射（IVF/ICSI）患者的滋养层测试整倍体囊胚（n=80）的 BFCM 样本中的 PAPP-A mRNA。BFCM 从囊胚期胚胎的 20 μL 液滴中获得。在囊胚玻璃化和 BFCM 收集用于冷冻之前，胚胎经历滋养层活检，以进行用于非整倍体的植入前基因检测。使用从 80 个单独的整倍体囊胚中收集的 BFCM 合成 cfDNA。接下来，进行实时 qPCR 以检测 PAPP-A 的表达，并用 GAPDH 对每个样本中的表达进行归一化。
在 80 个 BFCM 样本中的 45 个样本中检测到 PAPP-A mRNA（56.3%），样本之间表达水平存在差异。
我们的研究表明 PAPP-A 在 BFCM 中表达。据我们所知，这是第一项报道在 BFCM 中检测到 PAPP-A mRNA 的研究。需要进一步研究，并正在进行更多 BFCM 样本的研究，以及 PAPP-A 表达与转移的整倍体囊胚的妊娠结局的可能相关性。如果发现 PAPP-A 可以预测 IVF 和产科结局，那么它可能会与胚胎整倍性一起为胚胎转移提供额外的信息，以选择最佳的囊胚。
