Watkins D, Parsons S C, Cohen N
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, New York 14642.
Immunology. 1987 Dec;62(4):669-73.
Thymocytes from juevenile Xenopus laevis did not proliferate in response to commercial preparations of lipopolysaccharide (LPS), responded poorly when cultured with the T-cell mitogen, phytohaemagglutinin-P (PHA), and were not co-stimulated by PHA plus LPS. However, supernatants (SNs) from LPS-treated cultures of adult Xenopus macrophage-enriched resident peritoneal cells (PCs) enhanced the proliferative responses of thymocytes to a submitogenic dose of PHA. These SNs were incapable of supporting long-term growth of thymic lymphoblast cell lines, and thus could be distinguished from T-cell growth factor (TCGF)-rich SNs, which were essential for propagating these cells. The co-stimulatory activity was present in 0-24-hr SNs; after 48 hr, SN activity declined. No functional cross-reactivity of mammalian and Xenopus interleukin-1 (IL-1)-rich SNs was detected. These data are consistent with the proposition that a macrophage-derived factor, functionally homologous with mammalian IL-1, can enhance a T-cell proliferative response in an amphibian.
来自幼年非洲爪蟾的胸腺细胞不会因商业制备的脂多糖（LPS）而增殖，在用T细胞促有丝分裂原植物血凝素-P（PHA）培养时反应不佳，并且不会被PHA加LPS共刺激。然而，来自成年非洲爪蟾富含巨噬细胞的常驻腹膜细胞（PCs）经LPS处理的培养物的上清液（SNs）增强了胸腺细胞对亚致有丝分裂剂量PHA的增殖反应。这些SNs无法支持胸腺淋巴母细胞系的长期生长，因此可与富含T细胞生长因子（TCGF）的SNs区分开来，后者对这些细胞的增殖至关重要。共刺激活性存在于0 - 24小时的SNs中；48小时后，SN活性下降。未检测到哺乳动物和非洲爪蟾富含白细胞介素-1（IL-1）的SNs的功能交叉反应性。这些数据与以下观点一致，即一种与哺乳动物IL-1功能同源的巨噬细胞衍生因子可增强两栖动物的T细胞增殖反应。
