Bleicher P A, Rollins-Smith L A, Jacobs D M, Cohen N
Dev Comp Immunol. 1983 Summer;7(3):483-96. doi: 10.1016/0145-305x(83)90033-2.
We have compared in vitro mitogenic responses of frog (Xenopus laevis and Rana pipiens) lymphocytes to various preparations of lipopolysaccharide (LPS). Commercial LPS prepared from E. coli (phenol extraction) and from S. abortus-equi (phenol and TCA extraction procedures) was mitogenic for frog lymphocytes. After reextraction of these LPS preparations with phenol-water, the remaining LPS was either considerably less mitogenic or not mitogenic. Purified E. coli 055:B5 LPS, prepared by phenol water extraction, enzyme treatment and column chromatography, was not mitogenic. Frog cells proliferated poorly or not at all with all concentrations of reextracted or purified LPS tested (0.5-400 micrograms/ml) and at all culture periods examined (days 1-7). All LPS preparations used were mitogenic for CAF1 mouse lymphocytes, whereas reextracted and purified LPS preparations were not mitogenic for lymphocytes from C3H/HeJ cells. Xenopus were also not susceptible to toxicity induced by parenterally administered LPS in concentrations which killed CAF1 mice.
我们比较了青蛙（非洲爪蟾和豹蛙）淋巴细胞对各种脂多糖（LPS）制剂的体外促有丝分裂反应。从大肠杆菌（酚提取法）和马流产沙门氏菌（酚和三氯乙酸提取法）制备的市售LPS对青蛙淋巴细胞具有促有丝分裂作用。在用酚 - 水对这些LPS制剂进行再提取后，剩余的LPS促有丝分裂能力显著降低或无促有丝分裂作用。通过酚水提取、酶处理和柱色谱法制备的纯化大肠杆菌055:B5 LPS无促有丝分裂作用。在所测试的所有再提取或纯化LPS浓度（0.5 - 400微克/毫升）以及所有检测的培养时间段（第1 - 7天），青蛙细胞增殖不良或根本不增殖。所有使用的LPS制剂对CAF1小鼠淋巴细胞具有促有丝分裂作用，而重新提取和纯化的LPS制剂对C3H/HeJ细胞的淋巴细胞无促有丝分裂作用。非洲爪蟾对经肠胃外给予的能杀死CAF1小鼠的LPS浓度所诱导的毒性也不敏感。
