Vánky F, Roberts T, Klein E, Willems J
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Immunol Lett. 1987 Oct;16(1):21-6. doi: 10.1016/0165-2478(87)90055-1.
In a number of experiments performed with blood lymphocytes of patients, the high density subset lysed autologous tumor cells separated from the surgical specimens. The lysis was abrogated by pretreatment of the effector cells with antibodies (OKT3) directed against the T3 molecule associated with the T cell receptor and by pretreatment of the target cells with antibodies (W6/32) directed against the monomorphic part of the MHC class I antigens. This subset lyses only autologous tumor cells. The selectivity and the characteristics shared with antigen specific cytotoxic T lymphocytes (CTL) suggest that the auto-tumor lysis by the effectors reflects an immune response against the tumor cells. The low density lymphocytes, separated from the blood, can lyse both auto- and allogeneic tumor cell. In the autologous system, incubation of the effectors with the mAb OKT3 had no inhibitory effect and incubation of the targets with the anti W6/32 mAb inhibited their lysis only in some experiments. The nature of the reactivity of the LD lymphocytes remains to be defined. Whether it is similar to the indiscriminative natural killing or whether part of these lymphocytes are antigen(s) specific and exhibit a high avidity interaction with the target remains to be seen. It is possible that both types of target recognition occur since the LD lymphocyte population is heterogeneous.
在对患者血液淋巴细胞进行的多项实验中，高密度亚群可裂解从手术标本中分离出的自体肿瘤细胞。用针对与T细胞受体相关的T3分子的抗体（OKT3）预处理效应细胞，以及用针对MHC I类抗原单态部分的抗体（W6/32）预处理靶细胞，均可消除这种裂解作用。该亚群仅裂解自体肿瘤细胞。其选择性以及与抗原特异性细胞毒性T淋巴细胞（CTL）共有的特征表明，效应细胞对自体肿瘤的裂解反映了针对肿瘤细胞的免疫反应。从血液中分离出的低密度淋巴细胞可裂解自体和同种异体肿瘤细胞。在自体系统中，效应细胞与单克隆抗体OKT3孵育无抑制作用，而靶细胞与抗W6/32单克隆抗体孵育仅在某些实验中抑制其裂解。低密度淋巴细胞反应性的本质尚待确定。它是否类似于不加区分的自然杀伤作用，或者这些淋巴细胞中的一部分是否具有抗原特异性并与靶标表现出高亲和力相互作用，仍有待观察。由于低密度淋巴细胞群体是异质性的，两种类型的靶标识别都有可能发生。
