Hao Linjie, Wu Yanjie, Zhang Yumin, Ma Tao, Weng Pengfei, Zhang Binfei, Wang Junwei, Pei Qi
Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
J Clin Pharm Ther. 2022 Apr;47(4):517-522. doi: 10.1111/jcpt.13573. Epub 2022 Jan 10.
Up to now, no study focused on the role of SdrG in PJI after THA. To explore the mechanism of periprosthetic joint infection (PJI) after total hip arthroplasty (THA).
Joint fluid and blood were collected from patients with PJI after THA, aseptic loosening of the joints or bacterial infection after traumatic fractures of the extremities alone. The expression of SdrG in the 3 groups was determined by agarose gel electrophoresis. The expression of protein tyrosine phosphatase receptor J (PTPRJ) was measured by immunohistochemistry method. The platelet adhesion rate was determined by biochemical analysis. The content of D-dimer, CRP and ESR in blood was determined by latex agglutination method. Multiple linear correlation analysis was used to analyse the correlation between PJI and the expression of PTPRJ protein, platelet adhesion rate, D-dimer content, CRP and ESR.
The expression of SdrG and PTPRJ in PJI group was markedly increased compared to the other 2 groups. The platelet adhesion rate in PJI group was markedly larger compared to aseptic loosening and simple wound infection group, and the rate in simple wound infection group was larger than aseptic loosening group. The level of D-dimer, CRP and ESR in PJI group was higher than that of the other groups. The expression of PTPRJ protein, D-dimer content, CRP and ESR was all closely related to PJI, while platelet adhesion rate had no correlation with PJI.
SDRG gene around joint prosthesis was over-expressed, which activated joint surface membrane protein PTPRJ and then induced platelet aggregation to reduce joint function.
截至目前，尚无研究聚焦于SdrG在全髋关节置换术后假体周围关节感染（PJI）中的作用。旨在探究全髋关节置换术（THA）后假体周围关节感染的机制。
收集THA术后发生PJI、关节无菌性松动或单纯四肢创伤性骨折后细菌感染患者的关节液和血液。通过琼脂糖凝胶电泳测定3组中SdrG的表达。采用免疫组织化学方法检测蛋白酪氨酸磷酸酶受体J（PTPRJ）的表达。通过生化分析测定血小板黏附率。采用乳胶凝集法测定血液中D-二聚体、CRP和ESR的含量。运用多重线性相关分析分析PJI与PTPRJ蛋白表达、血小板黏附率、D-二聚体含量、CRP和ESR之间的相关性。
与其他2组相比，PJI组中SdrG和PTPRJ的表达显著增加。与无菌性松动组和单纯伤口感染组相比，PJI组的血小板黏附率显著更高，且单纯伤口感染组的血小板黏附率高于无菌性松动组。PJI组中D-二聚体、CRP和ESR的水平高于其他组。PTPRJ蛋白表达、D-二聚体含量、CRP和ESR均与PJI密切相关，而血小板黏附率与PJI无相关性。
假体周围关节处的SDRG基因过度表达，激活关节表面膜蛋白PTPRJ，进而诱导血小板聚集，导致关节功能下降。
