The Procter & Gamble Company, Mason Business Center, Mason, OH, USA.
P&G Singapore Innovation Center, Singapore City, Singapore.
J Eur Acad Dermatol Venereol. 2022 Feb;36 Suppl 3:3-11. doi: 10.1111/jdv.17846.
Hyperpigmented spots are common issues in all ethnicities, involving multiple intrinsic and extrinsic factors such as UVB exposure, hormone balance, inflammatory status and ageing.
To determine (i) melanocyte dendricity in multiple facial spot types, (ii) impact of High Mobility Group Box 1 (HMGB1), and the combination of sucrose dilaurate and sucrose laurate (SDL) on melanogenesis and melanocyte dendricity, and (iii) SDL effect on facial spots in a human use test.
Facial spot and adjacent non-spot skin biopsies were collected from Chinese women (age 20-70). Histological assessment of melanocyte dendricity was performed for 3 spot types (solar lentigo, melasma and postinflammatory hyperpigmentation) by immunofluorescent staining for c-kit/MITF. Keratinocyte, melanocyte and melanocyte-keratinocyte co-culture models were used to assess HMGB1 release by UVB radiation, the effects of HMGB1 and SDL on melanin production, melanocyte dendricity and melanosome transfer. The effect of an SDL-containing moisturizer on appearance of facial hyperpigmented spots was assessed against a vehicle control in an 8-week human use test.
Melanocytes in spot areas are more dendritic than melanocytes in adjacent non-spot skin across three investigated spot types. In cell culture models, a moderate UVB-radiation exposure caused release of HMGB1 from keratinocytes. HMGB1 did not alter melanin production in melanocytes, but enhanced melanocyte dendricity and melanosome transfer. SDL reduced HMGB1 release from keratinocytes, inhibited melanin production, reversibly suppressed melanocyte dendricity and reduced melanosome transfer. In the human use test, SDL-containing moisturizer reduced appearance of spots versus vehicle.
Increased melanocyte dendricity was observed in multiple types of facial spots. Addition of HMGB1 protein increased melanocyte dendricity and melanosome transfer in cell cultures, implicating potential involvement in spot formation. SDL suppressed melanin production, melanocyte dendricity and melanosome transfer in vitro and reduced appearance of spots in the use test, suggesting SDL is an effective solution to address hyperpigmented spot concerns.
色素沉着斑在所有种族中都很常见，涉及多种内在和外在因素，如 UVB 暴露、激素平衡、炎症状态和衰老。
确定(i)多种面部斑点类型的黑素细胞树突状，(ii)高迁移率族蛋白 B1（HMGB1）的影响，以及蔗糖月桂酸酯和蔗糖月桂酸酯（SDL）对黑素生成和黑素细胞树突状的影响，以及(iii)SDL 在人体使用试验中对面部斑点的影响。
从中国女性（年龄 20-70 岁）中采集面部斑点和相邻非斑点皮肤活检。通过免疫荧光染色 c-kit/MITF 对 3 种斑点类型（日光性雀斑样痣、黄褐斑和炎症后色素沉着过度）进行黑素细胞树突状的组织学评估。使用角质形成细胞、黑素细胞和黑素细胞-角质形成细胞共培养模型评估 UVB 辐射引起的 HMGB1 释放，HMGB1 和 SDL 对黑色素生成、黑素细胞树突状和黑素小体转移的影响。在 8 周的人体使用试验中，用含有 SDL 的保湿霜与载体对照评估其对面部色素沉着过度斑点外观的影响。
三种研究斑点类型中，斑点区域的黑素细胞比相邻非斑点皮肤的黑素细胞树突状更多。在细胞培养模型中，适度的 UVB 辐射会导致角质形成细胞释放 HMGB1。HMGB1 不会改变黑素细胞中的黑色素生成，但增强了黑素细胞树突状和黑素小体转移。SDL 减少了角质形成细胞中 HMGB1 的释放，抑制了黑色素的生成，可逆地抑制了黑素细胞树突状，并减少了黑素小体的转移。在人体使用试验中，含有 SDL 的保湿霜与载体相比减少了斑点的出现。
在多种类型的面部斑点中观察到黑素细胞树突状增加。HMGB1 蛋白的添加增加了细胞培养中的黑素细胞树突状和黑素小体转移，提示其可能参与了斑点的形成。SDL 在体外抑制黑色素生成、黑素细胞树突状和黑素小体转移，并减少使用试验中斑点的出现，表明 SDL 是解决色素沉着斑问题的有效方法。
