Tae Hyunhyuk, Park Soohyun, Ma Gamaliel Junren, Cho Nam-Joon
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore, Singapore.
China-Singapore International Joint Research Institute (CSIJRI), Guangzhou, 510000, China.
Nano Converg. 2022 Jan 11;9(1):3. doi: 10.1186/s40580-021-00292-5.
Cell-membrane-mimicking supported lipid bilayers (SLBs) provide an ultrathin, self-assembled layer that forms on solid supports and can exhibit antifouling, signaling, and transport properties among various possible functions. While recent material innovations have increased the number of practically useful SLB fabrication methods, typical SLB platforms only work in aqueous environments and are prone to fluidity loss and lipid-bilayer collapse upon air exposure, which limits industrial applicability. To address this issue, herein, we developed sucrose-bicelle complex system to fabricate air-stable SLBs that were laterally mobile upon rehydration. SLBs were fabricated from bicelles in the presence of up to 40 wt% sucrose, which was verified by quartz crystal microbalance-dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP) experiments. The sucrose fraction in the system was an important factor; while 40 wt% sucrose induced lipid aggregation and defects on SLBs after the dehydration-rehydration process, 20 wt% sucrose yielded SLBs that exhibited fully recovered lateral mobility after these processes. Taken together, these findings demonstrate that sucrose-bicelle complex system can facilitate one-step fabrication of air-stable SLBs that can be useful for a wide range of biointerfacial science applications.
细胞膜模拟支撑脂质双层（SLB）提供了一种超薄的自组装层，该层形成于固体支撑物上，在各种可能的功能中可表现出防污、信号传导和运输特性。虽然最近的材料创新增加了实用的SLB制造方法的数量，但典型的SLB平台仅在水性环境中起作用，并且在暴露于空气时容易出现流动性丧失和脂质双层塌陷的情况，这限制了其工业适用性。为了解决这个问题，在此我们开发了蔗糖双分子囊泡复合体系来制造空气稳定的SLB，这种SLB在再水化后具有横向流动性。SLB由双分子囊泡在高达40 wt%的蔗糖存在下制备而成，这通过石英晶体微天平耗散（QCM-D）和光漂白后荧光恢复（FRAP）实验得到了验证。体系中的蔗糖含量是一个重要因素；虽然40 wt%的蔗糖在脱水-再水化过程后会导致脂质聚集和SLB出现缺陷，但20 wt%的蔗糖产生的SLB在这些过程后表现出完全恢复的横向流动性。综上所述，这些发现表明蔗糖双分子囊泡复合体系可以促进空气稳定的SLB的一步制造，这对于广泛的生物界面科学应用可能是有用的。
