Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing, 401331, China.
Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, Chongqing University of Science and Technology, Chongqing, 401331, China.
Biomed Mater. 2022 Jan 25;17(2). doi: 10.1088/1748-605X/ac49f7.
Pure gelatin hydrogels lack antibacterial function and have poor mechanical properties, which restrict their application in wound dressings. In this study, nanosized silver bromide-doped mesoporous silica (AgBr@SiO) microspheres with hollow structures were prepared by a modified Stober method. The novel microspheres can not only release silver ions to treat bacteria but also release drugs to treat skin wound. Furthermore, AgBr@SiOmicrospheres were modified with propyl methacrylate, incorporated into methacrylated gelatin (GelMA), and crosslinked by UV light to prepare AgBr@SiO/GelMA dressings consisting of composite hydrogels. The results showed that the AgBr@SiOmicrospheres could enhance the mechanical properties of the hydrogels. With the increase in the AgBr@SiOconcentration from 0.5 to 1 mg ml, the dressings demonstrated effective antimicrobial activity against bothand. Furthermore, full-thickness skin woundswound healing studies with Sprague-Dawley rats were evaluated. When treated with AgBr@SiO/GelMA containing 1 mg mlAgBr@SiO, only 15% of the wound area left on day 10. Histology results also showed the epidermal and dermal layers were better organized. These results suggest that AgBr@SiO/GelMA-based dressing materials could be promising candidates for wound dressings.
纯明胶水凝胶缺乏抗菌功能，力学性能较差，限制了其在伤口敷料中的应用。本研究通过改进的 Stober 法制备了具有中空结构的纳米级银溴掺杂介孔硅（AgBr@SiO）微球。新型微球不仅可以释放银离子来治疗细菌，还可以释放药物来治疗皮肤伤口。此外，AgBr@SiO 微球用甲基丙烯酰丙基改性，掺入甲基丙烯酰化明胶（GelMA）中，并通过紫外光交联制备由复合水凝胶组成的 AgBr@SiO/GelMA 敷料。结果表明，AgBr@SiO 微球可以增强水凝胶的力学性能。随着 AgBr@SiO 浓度从 0.5 增加到 1mg/ml，敷料对和均表现出有效的抗菌活性。此外，还对 Sprague-Dawley 大鼠的全层皮肤伤口愈合进行了研究。当用含有 1mg/mlAgBr@SiO 的 AgBr@SiO/GelMA 处理时，第 10 天伤口面积仅留下 15%。组织学结果还表明表皮和真皮层组织更好。这些结果表明，基于 AgBr@SiO/GelMA 的敷料材料可能是伤口敷料的有前途的候选材料。
