Department of Laboratory Medicine, Yale School of Medicine, New Haven, Connecticut.
Lab Med. 2022 Sep 1;53(5):e105-e108. doi: 10.1093/labmed/lmab114.
To perform validation of plasma sodium on blood gas analyzers to reflexively correct erroneous measurements by ion-selective electrodes (ISEs).
We compared remnant specimens of whole blood and plasma collected by lithium heparin vacutainer with normal protein concentrations and no lipemia. Whole-blood specimens were tested for sodium concentration on the ABL800 Flex blood gas analyzer, followed by centrifugation for plasma separation, and repeat sodium determination on an aliquot of the plasma only. Also, plasma specimens were analyzed by indirect ISE on the Cobas 8000 series and by direct ISE on the ABL800 Flex for instrument comparison.
Plasma aliquots yielded comparable results to the parent whole-blood specimen, with an average change of -1.33 mmol/L (R2 = 0.9727). Comparison of indirect ISE to direct ISE similarly yielded comparable results, with an average change of + 0.8 mmol/L (R2 = 0.9016).
Plasma is a valid specimen matrix for use on blood gas analyzers for sodium determination, eliminating the need for re-collection of whole-blood specimens from patients with pseudohyponatremia.
对血气分析仪上的血浆钠进行验证，以通过离子选择性电极（ISEs）对错误的测量值进行反射性校正。
我们比较了锂肝素真空采血管采集的具有正常蛋白浓度且无脂血的全血和血浆剩余标本。使用 ABL800 Flex 血气分析仪对全血标本进行钠离子浓度检测，然后进行离心分离血浆，并仅对血浆的一份等分试样进行重复钠离子测定。此外，使用 Cobas 8000 系列间接 ISE 和 ABL800 Flex 直接 ISE 对血浆标本进行分析，以进行仪器比较。
血浆等分试样与原始全血标本具有可比性，平均变化为-1.33 mmol/L（R2=0.9727）。间接 ISE 与直接 ISE 的比较也产生了类似的可比结果，平均变化为+0.8 mmol/L（R2=0.9016）。
血浆是用于血气分析仪测定钠离子的有效标本基质，无需重新采集假性低钠血症患者的全血标本。
