Stenvinkel C, Sandberg G
Department of Histology, Karolinska Institute, Stockholm, Sweden.
J Clin Lab Immunol. 1987 Oct;24(2):87-91.
Normal human serum contains an IgM associated cytotoxic activity acting on guinea pig thymocytes. The inhibition of this cytotoxic effect by normal guinea pig serum was investigated. The blocking effect could be detected within 1 min and seemed to act by interfering with the cytotoxic factor in solution, since preincubation of human serum with autologous guinea pig serum potentiated the blocking effect, whereas no irreversible blocking effect was obtained by pre-incubation of target cells with the blocking factor. The inhibitory factor was not absorbed by incubation with thymocytes. The blocking effect was heat stabile (56 degrees C, 30 min) and detectable at both 4 degrees C and 37 degrees C. The clear effect on the cytotoxic effect of heterologous serum at 4 degrees C was in contrast to the much lower effect on the cytotoxicity of autologous serum at this temperature.
正常人血清含有一种作用于豚鼠胸腺细胞的IgM相关细胞毒性活性。研究了正常豚鼠血清对这种细胞毒性作用的抑制作用。阻断作用可在1分钟内检测到，似乎是通过干扰溶液中的细胞毒性因子起作用，因为人血清与自体豚鼠血清预孵育可增强阻断作用，而用阻断因子预孵育靶细胞未获得不可逆的阻断作用。抑制因子与胸腺细胞孵育后不被吸收。阻断作用对热稳定（56℃，30分钟），在4℃和37℃均可检测到。在4℃时对异源血清细胞毒性作用的明显影响与在该温度下对自体血清细胞毒性作用的低得多的影响形成对比。
