Nakazato T
Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan.
Neuroscience. 1987 Nov;23(2):529-38. doi: 10.1016/0306-4522(87)90074-1.
The intranuclear organization of the cat locus coeruleus neurons was investigated anatomo-physiologically. The locus coeruleus neurons project to the forebrain through the dorsal noradrenergic bundle and to the spinal cord. Horseradish peroxidase, a retrograde tracer, was pressure-injected into either the dorsal noradrenergic bundle or the ventrolateral funiculus of the high cervical cord (C1-C2). The cats (n = 12) were killed after a 2- or 3-day survival period. The frontal sections (100 micron) throughout the locus coeruleus were observed by light microscope after carrying out the diaminobenzidine reaction. The labeled locus coeruleus neurons were located predominantly in the rostral locus coeruleus proper and locus coeruleus alpha when horseradish peroxidase was injected into the dorsal noradrenergic bundle, whereas they were predominantly located in the caudal locus coeruleus alpha and subcoeruleus when horseradish peroxidase was injected into the spinal cord. In the electrophysiological experiments, cats (n = 30) were anesthetized with alpha-chloralose and two stimulating electrodes were placed stereotaxically in the dorsal noradrenergic bundle and the ipsilateral ventrolateral funiculus of the high cervical cord (C1-C2), respectively. Monophasic square-wave pulses (2.5 Hz, 100 microsecond duration, 800 microA) were delivered. A recording glass electrode, filled with 2 M NaCl saturated with Fast Green, was placed in the locus coeruleus. Neurons with different conduction velocities, which were evoked by the antidromic stimulation of the dorsal noradrenergic bundle and spinal cord, were verified in the locus coeruleus and the adjacent areas. The slow conductive neurons with a conduction velocity of less than 1 m/s had a slow firing rate (1.6 +/- 0.9/s). They were located predominantly in the rostral locus coeruleus proper and locus coeruleus alpha by the dorsal noradrenergic bundle stimulation. From the anatomical and electrophysiological experimental results, it was concluded that the conduction velocities of the horseradish peroxidase-labeled neurons observed in locus coeruleus proper and locus coeruleus alpha were mostly slow and less than 1 m/s. Most of the slow conductive neurons were considered to be noradrenergic. Neurons evoked antidromically by both the dorsal noradrenergic bundle and spinal cord stimulation were not observed.
采用解剖生理学方法研究了猫蓝斑核神经元的核内组织。蓝斑核神经元通过背侧去甲肾上腺素能束投射到前脑，并投射到脊髓。将逆行示踪剂辣根过氧化物酶经压力注射到背侧去甲肾上腺素能束或高颈段脊髓（C1 - C2）的腹外侧索中。在存活2或3天后处死猫（n = 12）。在进行二氨基联苯胺反应后，用光学显微镜观察贯穿蓝斑核的额叶切片（100微米）。当将辣根过氧化物酶注射到背侧去甲肾上腺素能束中时，标记的蓝斑核神经元主要位于吻侧蓝斑核本部和蓝斑核α区，而当将辣根过氧化物酶注射到脊髓中时，它们主要位于尾侧蓝斑核α区和蓝斑下核。在电生理实验中，用α - 氯醛糖麻醉猫（n = 30），并将两个刺激电极分别立体定位在背侧去甲肾上腺素能束和高颈段脊髓（C1 - C2）的同侧腹外侧索中。施加单相方波脉冲（2.5 Hz，持续时间100微秒，800微安）。将充满用固绿饱和的2 M NaCl的记录玻璃电极置于蓝斑核中。在蓝斑核及其相邻区域验证了由背侧去甲肾上腺素能束和脊髓的逆向刺激诱发的具有不同传导速度的神经元。传导速度小于1 m/s的慢传导神经元放电频率较慢（1.6±0.9 /秒）。通过背侧去甲肾上腺素能束刺激，它们主要位于吻侧蓝斑核本部和蓝斑核α区。从解剖学和电生理实验结果得出结论，在蓝斑核本部和蓝斑核α区观察到的辣根过氧化物酶标记神经元的传导速度大多较慢，小于1 m/s。大多数慢传导神经元被认为是去甲肾上腺素能的。未观察到由背侧去甲肾上腺素能束和脊髓刺激均逆向诱发的神经元。
