Kriebel M E, Motelica-Heino I
Department of Physiology, State University of New York, Health Science Center at Syracuse 13210.
Neuroscience. 1987 Nov;23(2):757-66. doi: 10.1016/0306-4522(87)90093-5.
Miniature endplate potentials were recorded from the isolated pectoralis cutaneous muscle of the frog during very stable recording conditions and low noise levels. Two to 5 x 10(3) miniature endplate potentials were filmed at eight unstressed junctions that met rigorous experimental criteria for analysis. Seven junctions generated enough sub-miniature endplate potentials (1-3%) to produce a bell-shaped amplitude distribution. The sub-miniature endplate potential means were usually 9-10 times smaller than the modal bell-miniature endplate potential values. The standard deviation of the sub-miniature endplate potential class was calculated by subtracting the noise and measurement error from the measured sub-miniature endplate potential distribution. The coefficients of variation of the sub-miniature endplate potential distribution were 9-16%. Half of the bell-miniature endplate potential amplitude distributions showed 4-6 integral peaks in the central part of the distributions and the positions of these peaks were maintained with increasing sample size. The remaining distributions were not smooth and suggested integral peaks. The intervals between the peaks were about the same size as the sub-miniature endplate potential mode. These data provide further evidence for the subunit hypothesis of the quantum of transmitter release and describe the amplitude distribution of the sub-miniature endplate potential class of the adult preparation.
在非常稳定的记录条件和低噪声水平下，从青蛙分离的胸皮肌记录微小终板电位。在八个符合严格分析实验标准的无应力接头处拍摄了2至5×10³个微小终板电位。七个接头产生了足够的亚微小终板电位（1 - 3%），以产生钟形幅度分布。亚微小终板电位平均值通常比典型的钟形微小终板电位值小9至10倍。通过从测量的亚微小终板电位分布中减去噪声和测量误差来计算亚微小终板电位类别的标准偏差。亚微小终板电位分布的变异系数为9 - 16%。一半的钟形微小终板电位幅度分布在分布中心部分显示出4至6个积分峰，并且随着样本量增加这些峰的位置保持不变。其余分布不光滑并暗示有积分峰。峰之间的间隔与亚微小终板电位模式大小大致相同。这些数据为递质释放量子的亚基假说提供了进一步证据，并描述了成年制剂中亚微小终板电位类别的幅度分布。
