Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan.
Division of Carcinogenesis, The Cancer Institute, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo 135-8550, Japan.
Lab Chip. 2022 Feb 1;22(3):641-651. doi: 10.1039/d1lc00751c.
Three-dimensional (3D) tissue culture is a powerful tool for understanding physiological events. However, 3D tissues still have limitations in their size, culture period, and maturity, which are caused by the lack of nutrients and oxygen supply through the vasculature. Here, we propose a new method for culturing a 3D tissue-a spheroid-directly on an 'on-chip vascular bed'. The method can be applied to any 3D tissue because the vascular bed is preformed, so that angiogenic factors from the tissue are not necessary to induce vasculature. The essential component of the assay system is the removable membrane that initially separates the 3D tissue culture well and the microchannel in which a uniform vascular bed is formed, and then allows the tissue to be settled directly onto the vascular bed following its removal. This system offers a new technique for evaluating the effects of vasculature on 3D tissues.
三维（3D）组织培养是理解生理事件的有力工具。然而，3D 组织在其大小、培养期和成熟度方面仍然存在局限性，这是由于缺乏通过脉管系统供应的营养和氧气。在这里，我们提出了一种在“芯片上血管床”上直接培养 3D 组织-球体的新方法。该方法可适用于任何 3D 组织，因为血管床是预先形成的，因此不需要组织中的血管生成因子来诱导血管形成。该分析系统的基本组成部分是可移动的膜，该膜最初将 3D 组织培养孔与形成均匀血管床的微通道隔开，然后在去除该膜后，允许组织直接沉降到血管床上。该系统为评估血管对 3D 组织的影响提供了一种新技术。
