Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Professor of Periodontology, Department of Periodontology - Division of Dentistry Research, University of Guarulhos, Guarulhos, São Paulo, Brazil.
Braz Dent J. 2021 Nov-Dec;32(6):66-73. doi: 10.1590/0103-6440202104533.
The aim of the present in vivo study was to evaluate the bacterial contamination of sports mouthguards, surface roughness, and the efficacy of chlorhexidine gluconate spray in the disinfection of these devices. A randomized, blinded cross-over clinical trial was performed with twenty 9 to 13 years old children who practiced martial arts and participated in all phases of the study. They were instructed to wear mouthguards 3 alternated days a week for 1 hour and, after use, to spray sterile tap water or chlorhexidine 0.12%. The mouthguards were analyzed by MTT assay, Checkerboard DNA-DNA hybridization, and confocal laser microscopy prior and after use for 2 weeks. Data were analyzed by Wilcoxon and t-Student, and Pearson correlation tests, with 5% significance level. Were observed that mouthguards of the control group were more contaminated with cariogenic microorganisms than those of the chlorhexidine group (p<0.05). The mouthguards use of spray of chlorhexidine reduced significantly the bacteria contamination compared with control group (p = 0.007). The surface roughness of the mouthguards increased significantly after use, irrespective of application of chlorhexidine spray. A moderate correlation (r=0.59) was observed between surface roughness and the cariogenic microorganism's contamination only for control group. Sports mouthguards had intense microbial contamination and increased surface roughness after its use. The use of chlorhexidine spray was effective for reducing the mouthguards contamination used by children.
本体内研究旨在评估运动护齿器的细菌污染、表面粗糙度以及葡萄糖酸氯己定喷雾在这些器械消毒中的效果。采用随机、双盲交叉临床试验，纳入 20 名 9 至 13 岁、练习武术并参与研究所有阶段的儿童。他们被指示每 3 天交替佩戴护齿器 1 小时，使用后用无菌自来水或 0.12%葡萄糖酸氯己定喷雾器喷洒。在使用前和使用后 2 周，通过 MTT 测定法、棋盘 DNA-DNA 杂交和共聚焦激光显微镜分析护齿器。采用 Wilcoxon 和 t-Student 以及 Pearson 相关检验分析数据，置信度水平为 5%。结果表明，对照组的护齿器比葡萄糖酸氯己定组更容易被致龋微生物污染（p<0.05）。与对照组相比，葡萄糖酸氯己定喷雾器的使用显著降低了细菌污染（p = 0.007）。护齿器使用后，表面粗糙度显著增加，而不论是否应用葡萄糖酸氯己定喷雾器。仅在对照组中，观察到表面粗糙度与致龋微生物污染之间存在中度相关性（r=0.59）。运动护齿器在使用后会受到严重的微生物污染，并增加表面粗糙度。使用葡萄糖酸氯己定喷雾器可有效减少儿童使用的护齿器污染。
