Nicklin Matthew, Hickman Graham J, Pockley A Graham, Perry Carole C
ACS Appl Bio Mater. 2020 Jan 21;3(1):495-504. doi: 10.1021/acsabm.9b00940. Epub 2020 Jan 7.
OPCT-1 is a heterogeneous prostate cancer cell line derived from primary (rather than metastatic) disease which contains epithelial, mesenchymal, and CD44/CD24 cancer stem cell (CSC) subpopulations and from which we have previously generated and characterized stable mesenchymal (P4B6B) and epithelial (P5B3) cell subpopulations. In this contribution, we explore the effect of tissue culture surface chemistry (standard tissue culture plastic (TCP) and a fluoroalkylsilica (FS) culture surface with inherently low surface energy) on the phenotype and adherent capacity of mesenchymal and epithelial cell populations. We demonstrate that OPCT-1 cells adherent to FS surfaces comprise both epithelial- and mesenchymal-like populations; a mesenchymal subpopulation derived from OPCT1 (P4B6B) poorly adheres to FS and formed spheroids, whereas an epithelial subpopulation derived from OPCT1 (P5B3) forms an adherent monolayer. In contrast, P4B6B cells do adhere to FS when cocultured with P5B3 cells. Taken together, these findings demonstrate that EMT/cell differentiation status dictates cell adhesive capacity and provide a novel insight into the relationship between epithelial and mesenchymal cell populations in metastasis. Importantly, the differences in adherence capacity between P4B6B and P5B3 are not apparent using standard TCP-based culture, thereby highlighting the value of using alternative culture surfaces for studying cell surface interaction/adhesion phenomena and interrogating mechanisms involved in adhesion and detachment of metastatic tumor cells.
OPCT-1是一种源自原发性（而非转移性）疾病的异质性前列腺癌细胞系，它包含上皮、间充质和CD44/CD24癌症干细胞（CSC）亚群，我们之前已从该细胞系中分离并鉴定出稳定的间充质（P4B6B）和上皮（P5B3）细胞亚群。在本研究中，我们探讨了组织培养表面化学性质（标准组织培养塑料（TCP）和具有固有低表面能的氟代烷基硅烷（FS）培养表面）对间充质和上皮细胞群体表型及黏附能力的影响。我们证明，黏附于FS表面的OPCT-1细胞包含上皮样和间充质样群体；源自OPCT1的间充质亚群（P4B6B）在FS上黏附性差并形成球体，而源自OPCT1的上皮亚群（P5B3）则形成黏附单层。相比之下，当与P5B3细胞共培养时，P4B6B细胞确实能黏附于FS。综上所述，这些发现表明上皮-间质转化/细胞分化状态决定细胞黏附能力，并为转移过程中上皮和间充质细胞群体之间的关系提供了新的见解。重要的是，使用基于标准TCP的培养方法时，P4B6B和P5B3之间的黏附能力差异不明显，从而突出了使用替代培养表面研究细胞表面相互作用/黏附现象以及探究转移性肿瘤细胞黏附和解离所涉及机制的价值。
