Richardson B C, Laing T J, Cease K B
Department of Internal Medicine, University of Michigan, Ann Arbor 48109.
Immunol Invest. 1987 Nov;16(7):589-605. doi: 10.3109/08820138709087104.
The T cell-macrophage interaction, necessary for T cell activation, has nonspecific and specific components. Nonspecific T-macrophage interactions are mediated by surface glycoproteins such as LFA 1, 2 and 3, while specific interactions are mediated by the T3-Ti complex on the T cell and antigen plus Ia molecules on the macrophage. To determine the relative contributions of specific antigen to the total avidity of T-macrophage binding we adapted a novel assay capable of providing quantitative estimations of the avidity of cell-cell interactions. Using this assay we determined the avidity of a cloned CD4+ antigen specific T cell line for autologous macrophages with and without antigen. The presence of specific antigen increased binding avidity by approximately 25%. This assay should prove useful in further characterizing the avidity of T-macrophage interactions.
T细胞激活所必需的T细胞与巨噬细胞的相互作用具有非特异性和特异性成分。非特异性T细胞与巨噬细胞的相互作用由诸如淋巴细胞功能相关抗原1、2和3等表面糖蛋白介导，而特异性相互作用则由T细胞上的T3-Ti复合物以及巨噬细胞上的抗原加Ia分子介导。为了确定特异性抗原对T细胞与巨噬细胞结合的总亲和力的相对贡献，我们采用了一种能够对细胞间相互作用的亲和力进行定量估计的新检测方法。使用该检测方法，我们测定了一个克隆的CD4+抗原特异性T细胞系与有或没有抗原的自体巨噬细胞的结合亲和力。特异性抗原的存在使结合亲和力提高了约25%。该检测方法在进一步表征T细胞与巨噬细胞相互作用的亲和力方面应会被证明是有用的。
