Sun Cheng-He, Zhang Ya-Nan, Zeng Xiao-Shu, Liu Da-Wei, Huang Qi, Zhang Xiao-Li, Zhang Qun
Department of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, 510632, China.
Forest Police Identification Center of National Forestry Administration, Nanjing Forest Police College, Nanjing, 210023, China.
Mol Biol Rep. 2022 Mar;49(3):1741-1748. doi: 10.1007/s11033-021-06983-w. Epub 2022 Jan 13.
The taxonomic status of Knodu in the family Characidae is not yet clear. This study aimed to address this by sequencing and annotating Knodu borki Zarske, 2008.
K. borki Zarske, 2008 was sequenced using a Hiseq platform and the complete mitogenome was assembled in SPAdes v3.15.2 and SOAPdenovo2 v.2.01. The mitogenome of K. borki from Guangzhou, the first sequenced species of the genus Knodu, is 16,837 bp in length and contains 13 protein-coding genes (PCGs), two ribosomal (r) RNAs, 22 transfer (t) RNAs, and one D-loop. Among these 37 genes, 28 are encoded by the heavy strand, while nine are encoded by the light strand. Twenty-one of the tRNAs can form typical cloverleaf secondary structures, except tRNA-Ser1, which lacks dihydrouridine arms. All PCGs have the same start codon (ATG), with the exception of COI (GTG). Four PCGs (ND1, ATP8, ND4L, and ND5) have TAA as the stop codon, ND6 has TAG as the stop codon, COI has AGG as the stop codon, and the remaining seven genes have incomplete stop codons of TA-/T-(ND2, COII, COIII, ND3, ND4, and Cyt b as T-, ATP6 as TA-). Phylogenetic analysis showed that K. borki belongs to the family Characidae.
Our findings demonstrate that K. borki belongs to the family Characidae, due to consistency with the morphological identification. This study provides molecular information for further research on the phylogeny of the genus Knodus and for analyses of the taxonomic status of Characidae.
脂鲤科中克氏脂鲤属的分类地位尚不清楚。本研究旨在通过对2008年的博氏克氏脂鲤进行测序和注释来解决这一问题。
使用Hiseq平台对2008年的博氏克氏脂鲤进行测序，并在SPAdes v3.15.2和SOAPdenovo2 v.2.01中组装完整的线粒体基因组。来自广州的博氏克氏脂鲤的线粒体基因组是该属第一个测序物种，长度为16,837 bp，包含13个蛋白质编码基因（PCGs）、两个核糖体（r）RNA、22个转运（t）RNA和一个D环。在这37个基因中，28个由重链编码，9个由轻链编码。除缺乏二氢尿苷臂的tRNA-Ser1外，21个tRNA可形成典型的三叶草二级结构。所有PCGs都有相同的起始密码子（ATG），COI除外（GTG）。四个PCGs（ND1、ATP8、ND4L和ND5）以TAA作为终止密码子，ND6以TAG作为终止密码子，COI以AGG作为终止密码子，其余七个基因具有不完整的TA-/T-终止密码子（ND2、COII、COIII、ND3、ND4和Cyt b为T-，ATP6为TA-）。系统发育分析表明，博氏克氏脂鲤属于脂鲤科。
我们的研究结果表明，由于与形态学鉴定一致，博氏克氏脂鲤属于脂鲤科。本研究为进一步研究克氏脂鲤属的系统发育和分析脂鲤科的分类地位提供了分子信息。
