Filina Anya, Yousefi Nariman, Okshevsky Mira, Tufenkji Nathalie
Department of Chemical Engineering, McGill University, 3610 University Street, Montréal, Québec H3A 0C5, Canada.
ACS Appl Bio Mater. 2019 Apr 15;2(4):1578-1590. doi: 10.1021/acsabm.9b00008. Epub 2019 Mar 18.
Water treatment technologies based on graphene oxide (GO) sponges show promise due to their high surface area and versatile chemistry, yielding an excellent adsorption affinity for different contaminants. However, the bacteria removal capacity and the intrinsic antimicrobial properties of GO sponges are not well understood. While GO has been successfully functionalized with antibiotics or metal biocides, these antimicrobials present cytotoxicity concerns. Natural antimicrobial agents such as antimicrobial enzymes, peptides, and polymers hold promise in this respect due to their relatively low cost, biocompatibility, and ability to readily functionalize GO by covalent bond formation with oxygen-containing functional groups. In this work, the antimicrobial enzyme lysozyme, antimicrobial peptide nisin, and antimicrobial polyamide ε-poly-l-lysine were used to covalently functionalize the surface of a hierarchically porous GO sponge. The antimicrobial activity of the functionalized material was demonstrated against two model organisms: the Gram-positive and Gram-negative . The performance of the porous material in a simulated filtration context was evaluated using packed column experiments, and an improved bacterial retention of both strains by the functionalized sponge was demonstrated. Furthermore, samples of spent sponge after filtration were evaluated with a membrane integrity assay demonstrating antimicrobial activity in a continuous flow mode.
基于氧化石墨烯（GO）海绵的水处理技术因其高表面积和多样的化学性质而展现出前景，对不同污染物具有出色的吸附亲和力。然而，GO海绵的细菌去除能力和内在抗菌性能尚未得到充分了解。虽然GO已成功用抗生素或金属杀菌剂进行功能化，但这些抗菌剂存在细胞毒性问题。天然抗菌剂如抗菌酶、肽和聚合物在这方面具有潜力，因为它们成本相对较低、具有生物相容性，并且能够通过与含氧官能团形成共价键而容易地对GO进行功能化。在这项工作中，抗菌酶溶菌酶、抗菌肽乳链菌肽和抗菌聚酰胺ε-聚-L-赖氨酸被用于对分级多孔GO海绵表面进行共价功能化。对功能化材料针对两种模式生物（革兰氏阳性菌和革兰氏阴性菌）的抗菌活性进行了验证。使用填充柱实验评估了多孔材料在模拟过滤环境中的性能，结果表明功能化海绵对两种菌株的细菌截留能力有所提高。此外，通过膜完整性测定对过滤后用过的海绵样品进行了评估，证明其在连续流动模式下具有抗菌活性。
