Department of Anesthesiology, Ganzhou People's Hospital, No.16, Meiguan Avenue, Zhanggong District, Ganzhou City, 341000, Jiangxi Province, China.
Metab Brain Dis. 2022 Mar;37(3):787-799. doi: 10.1007/s11011-022-00906-9. Epub 2022 Jan 15.
Sevoflurane (SEV) is a common anesthetic to inhibit glioma progression. The previous studies have indicated the molecular mechanisms of SEV function in glioma. The objective of this study was to explore the association of circ_00037655 with SEV in glioma. Cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was analyzed using Edu assay and colony formation assay. Flow cytometry was applied to determine cell apoptosis. Protein analysis was performed via western blot. Cell migration and invasion were assessed by transwell assay. Circ_0037655, microRNA-130a-5p (miR-130a-5p) and ribophorin II (RPN2) levels were detected using the quantitative real-time polymerase chain reaction (qRT-PCR). Dual-luciferase reporter, RNA immunoprecipitation (RIP) and pull-down assays were used to analyze target interaction. The effect of circ_0037655 on SEV in vivo was researched by xenograft models. SEV reduced cell viability, proliferation, migration and invasion but induced apoptosis of glioma cells. Circ_0037655 expression was inhibited after SEV treatment in glioma cells. The effects of SEV on glioma cell behaviors were attenuated by upregulation of circ_0037655. Circ_0037655 interacted with miR-130a-5p and miR-130a-5p targeted RPN2. Circ_0037655 or miR-130a-5p regulated the anti-tumor function of SEV in glioma by targeting miR-130a-5p or RPN2. Circ_0037655 affected the expression of RPN2 via targeting miR-130a-5p. Circ_0037655 relieved SEV-induced glioma growth inhibition in vivo by mediating miR-130a-5p and RPN2 levels. SEV inhibited the malignant progression of glioma cells partly by regulating the circ_0037655/miR-130a-5p/RPN2 axis.
七氟醚（SEV）是一种抑制神经胶质瘤进展的常用麻醉剂。先前的研究表明了 SEV 在神经胶质瘤中的作用的分子机制。本研究旨在探讨 circ_00037655 与 SEV 在神经胶质瘤中的关联。通过细胞计数试剂盒（CCK-8）测定细胞活力。通过 EdU 测定和集落形成测定分析细胞增殖。通过流式细胞术测定细胞凋亡。通过 Western blot 进行蛋白质分析。通过 Transwell 测定评估细胞迁移和侵袭。使用实时定量聚合酶链反应（qRT-PCR）检测 circ_0037655、微小 RNA-130a-5p（miR-130a-5p）和核糖体蛋白 II（RPN2）水平。使用双荧光素酶报告基因、RNA 免疫沉淀（RIP）和下拉测定分析靶标相互作用。通过异种移植模型研究 circ_0037655 对 SEV 的体内影响。SEV 降低神经胶质瘤细胞的活力、增殖、迁移和侵袭，但诱导细胞凋亡。SEV 处理后神经胶质瘤细胞中 circ_0037655 的表达受到抑制。上调 circ_0037655 减弱了 SEV 对神经胶质瘤细胞行为的影响。Circ_0037655 与 miR-130a-5p 相互作用，miR-130a-5p 靶向 RPN2。Circ_0037655 或 miR-130a-5p 通过靶向 miR-130a-5p 或 RPN2 调节 SEV 在神经胶质瘤中的抗肿瘤作用。Circ_0037655 通过靶向 miR-130a-5p 影响 RPN2 的表达。Circ_0037655 通过调节 miR-130a-5p 和 RPN2 水平缓解 SEV 诱导的体内神经胶质瘤生长抑制。SEV 通过调节 circ_0037655/miR-130a-5p/RPN2 轴部分抑制神经胶质瘤细胞的恶性进展。
