抗2'-甲基核糖核酸寡聚物抑制丝状化并减轻念珠菌病。
Anti- 2'-MethylRNA oligomer inhibits filamentation and attenuates the candidiasis in .
作者信息
Araújo Daniela, Mil-Homens Dalila, Henriques Mariana, Silva Sónia
机构信息
LIBRO - Laboratório de Investigação em Biofilmes Rosário Oliveira, CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Lisbon University, 1049-001 Lisbon, Portugal.
出版信息
Mol Ther Nucleic Acids. 2021 Dec 16;27:517-523. doi: 10.1016/j.omtn.2021.12.018. eCollection 2022 Mar 8.
is a central transcriptional regulator of filamentation that is an important virulence factor of . This study serves to assess the applicability of the anti- 2'-MethylRNA oligomer for inhibiting filamentation and to attenuate candidiasis, using the model. For that, larvae infected with a lethal concentration of cells were treated with a single dose and with a double dose of the anti- 2'Me oligomer (at 40 and 100 nM). The anti- 2'OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti- 2'OMe oligomer toxicity was observed and the treatment with double dose of 2'OMe oligomer empowered larvae survival over 24 h (by 90%-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the therapeutic potential of anti- 2'Me oligomer for controlling infections.
是丝状化的核心转录调节因子，是一种重要的毒力因子。本研究旨在评估抗2'-甲基核糖核酸低聚物抑制丝状化并减轻念珠菌病的适用性，采用模型进行研究。为此，用致死浓度的细胞感染幼虫，并用单剂量和双剂量的抗2'-甲基寡聚物（40和100 nM）进行处理。评估了抗2'-甲氧基寡聚物的毒性及其对幼虫存活的影响。未观察到抗2'-甲氧基寡聚物毒性的证据，双剂量的2'-甲氧基寡聚物处理可使幼虫在24小时内的存活率提高（90%-100%），并将其疗效延长至感染后72小时（提高30%）。毫无疑问，这项工作验证了抗2'-甲基寡聚物在控制感染方面的治疗潜力。
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