Synthesis, Bioactivity Assessment, and Molecular Docking of Non-sulfonamide Benzimidazole-Derived -Acylhydrazone Scaffolds as Carbonic Anhydrase-II Inhibitors.

This research reports the synthesis of new benzimidazole-derived -acylhydrazones (NAH), their characterization using various spectroscopic methods, and in vitro evaluation as potent carbonic anhydrase-II inhibitors. Among the target compounds (), few showed higher inhibition than the standard acetazolamide (IC: 18.6 ± 0.43 μM), for example, compound (IC: 13.3 ± 1.25 μM), (IC: 17.2 ± 1.24 μM), (IC: 14.6 ± 0.62 μM), and (IC: 14.5 ± 1.05 μM). Molecular docking was performed on the most active compounds, which revealed their binding interactions with the active site of the enzyme, thus supporting the experimental findings.