Department of Electrical Engineering and Information Systems, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
J Phys Chem B. 2022 Jan 27;126(3):708-715. doi: 10.1021/acs.jpcb.1c09941. Epub 2022 Jan 18.
Alteration of the hydrogen-bond (H-bond) network by trehalose is acknowledged as a bioprotective agent. However, most studies exploring the hydration superiority of the trehalose structure are limited structure are limited by the computational cost or a narrow-range spectrum. In the present study, the structural and dynamical behaviors of the H-bond network of trehalose and maltose solutions were observed and compared with a broadband dielectric spectrum (100 MHz-18 THz) to investigate the influence of the trehalose structure on the bioprotective function. From the relaxation time, the reorientation cooperativity, resonant frequency, and damping constant of water-water vibration, the symmetric structure of trehalose allowed a more significant H-bond strengthening effect and homogeneous aqueous environment. In contrast, the difference in the hydration number between trehalose and maltose was negligible. Thus, the enhanced H-bond strengthening effect and homogeneous aqueous environment owing to the symmetric structure are the essential factors that contribute to the remarkable bioprotective effect of trehalose.
海藻糖通过改变氢键（H-bond）网络被认为是一种生物保护剂。然而，大多数探索海藻糖结构优势的水化作用的研究受到计算成本或窄范围谱的限制。在本研究中，观察了海藻糖和麦芽糖溶液中氢键网络的结构和动力学行为，并与宽带介电谱（100 MHz-18 THz）进行了比较，以研究海藻糖结构对生物保护功能的影响。从弛豫时间、取向协同性、共振频率和水-水振动的阻尼常数来看，海藻糖的对称结构允许更显著的氢键强化效应和均匀的水相环境。相比之下，海藻糖和麦芽糖的水合数差异可以忽略不计。因此，由于对称结构而增强的氢键强化效应和均匀的水相环境是海藻糖具有显著生物保护效果的重要因素。
