Wolf B
Institut für Immunbiologie, Universität Freiburg/Brsg., Federal Republic of Germany.
J Electron Microsc Tech. 1987 Nov;7(3):185-9. doi: 10.1002/jemt.1060070306.
Cryoultramicrotomy is gaining in significance and use as technical improvements are made. In comparison with epoxy resin procedures, cryopreparations are more rapid; there is only a negligible loss of material and thus antigenicity is well preserved. The present work contains some newly developed details for the exact determination of the advancement of the sample in the direction of the knife in a microtome. It is a software-controlled ultramicrotome advance drive system with feedback regulation as well as repeatedly patented devices for advancement sensors.
随着技术的改进，冷冻超薄切片术在重要性和应用方面都在不断提高。与环氧树脂切片法相比，冷冻切片制备速度更快；材料损失可忽略不计，因此抗原性得到了很好的保留。本研究包含了一些新开发的细节，用于精确确定切片机中样品向刀的推进方向。它是一个软件控制的超薄切片机推进驱动系统，具有反馈调节功能，以及多次获得专利的推进传感器装置。
