Department of Microbiology, School of Biological Sciences, Central University of Punjab, Bathinda, Punjab, India.
Department of Zoology, School of Biological Sciences, Central University of Punjab, Bathinda, Punjab, India.
Methods Mol Biol. 2022;2413:69-76. doi: 10.1007/978-1-0716-1896-7_9.
Oxidative stress has been implicated in various human diseases, including cancer, mainly through the generation of reactive nitrogen species (RNS), such as nitric oxide (NO), nitrite, nitroxyl, s-nitrosothiols, and reactive oxygen species (ROS) such as peroxides, superoxide, and hydroxyl radicals. NO being the main player among RNS induced altered cellular molecules and metabolisms, thus making it important to understand and detect the generation of NO in biological samples. There are many methods for direct and indirect detection of NO; out of these most commonly used are spectrophotometric-based Griess assay and fluorescence probe-based assays. In this chapter, we summarize these routinely used methods to detect NO and various challenges associated with these methods.
氧化应激与包括癌症在内的多种人类疾病有关，主要是通过生成活性氮物种（RNS），如一氧化氮（NO）、亚硝酸盐、硝普盐、S-亚硝基硫醇和活性氧物种（ROS），如过氧化物、超氧化物和羟自由基。NO 是 RNS 诱导的细胞分子和代谢改变的主要参与者，因此了解和检测生物样本中 NO 的生成非常重要。有许多直接和间接检测 NO 的方法；其中最常用的是基于分光光度法的格里斯测定法和基于荧光探针的测定法。在本章中，我们总结了这些常用的检测 NO 的方法以及这些方法相关的各种挑战。
