• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

PKD2L1 通道显性负性表达的小鼠β-肾上腺素能反应增强。

Enhanced β-adrenergic response in mice with dominant-negative expression of the PKD2L1 channel.

机构信息

Department of Pharmacology, Faculty of Medicine, University of Miyazaki, Miyazaki, Miyazaki, Japan.

Department of Pharmacology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.

出版信息

PLoS One. 2022 Jan 20;17(1):e0261668. doi: 10.1371/journal.pone.0261668. eCollection 2022.

DOI:10.1371/journal.pone.0261668
PMID:35051185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8775249/
Abstract

Polycystic kidney disease (PKD) is the most common genetic cause of kidney failure in humans. Among the various PKD-related molecules, PKD2L1 forms cation channels, but its physiological importance is obscure. In the present study, we established a transgenic mouse line by overexpressing the dominant-negative form of the mouse PKD2L1 gene (i.e., lacking the pore-forming domain). The resulting PKD2L1del-Tg mice exhibited supraventricular premature contraction, as well as enhanced sensitivity to β-adrenergic stimulation and unstable R-R intervals in electrocardiography. During spontaneous atrial contraction, PKD2L1del-Tg atria showed enhanced sensitivity to isoproterenol, norepinephrine, and epinephrine. Action potential recording revealed a shortened action potential duration in PKD2L1del-Tg atria in response to isoproterenol. These findings indicated increased adrenergic sensitivity in PKD2L1del-Tg mice, suggesting that PKD2L1 is involved in sympathetic regulation.

摘要

多囊肾病（PKD）是人类肾衰竭的最常见遗传原因。在各种 PKD 相关分子中，PKD2L1 形成阳离子通道，但它的生理重要性尚不清楚。在本研究中，我们通过过表达小鼠 PKD2L1 基因的显性失活形式（即缺乏形成孔的结构域）建立了转基因小鼠系。由此产生的 PKD2L1del-Tg 小鼠表现出室上性期前收缩，以及对β-肾上腺素刺激的敏感性增强和心电图中不稳定的 R-R 间隔。在自发性心房收缩期间，PKD2L1del-Tg 心房对异丙肾上腺素、去甲肾上腺素和肾上腺素的敏感性增强。动作电位记录显示 PKD2L1del-Tg 心房对异丙肾上腺素的反应中动作电位持续时间缩短。这些发现表明 PKD2L1del-Tg 小鼠的肾上腺素敏感性增加，表明 PKD2L1 参与了交感神经调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/22edaf8f15a1/pone.0261668.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/0bbcdf8ec421/pone.0261668.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/71e8d2026214/pone.0261668.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/3aaf7badcd50/pone.0261668.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/54940f8c4eed/pone.0261668.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/22edaf8f15a1/pone.0261668.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/0bbcdf8ec421/pone.0261668.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/71e8d2026214/pone.0261668.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/3aaf7badcd50/pone.0261668.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/54940f8c4eed/pone.0261668.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1071/8775249/22edaf8f15a1/pone.0261668.g005.jpg

相似文献

1
Enhanced β-adrenergic response in mice with dominant-negative expression of the PKD2L1 channel.PKD2L1 通道显性负性表达的小鼠β-肾上腺素能反应增强。
PLoS One. 2022 Jan 20;17(1):e0261668. doi: 10.1371/journal.pone.0261668. eCollection 2022.
2
Modified sympathetic nerve system activity with overexpression of the voltage-dependent calcium channel beta3 subunit.电压依赖性钙通道β3亚基过表达导致的交感神经系统活动改变。
J Biol Chem. 2008 Sep 5;283(36):24554-60. doi: 10.1074/jbc.M802319200. Epub 2008 Jul 15.
3
Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice.钙/钙调蛋白依赖性蛋白激酶IV基因敲除小鼠中β-肾上腺素能反应减弱
PLoS One. 2021 Apr 15;16(4):e0249932. doi: 10.1371/journal.pone.0249932. eCollection 2021.
4
Cardiostimulant and cardiodepressant effects through overexpressed human beta2-adrenoceptors in murine heart: regional differences and functional role of beta1-adrenoceptors.通过在小鼠心脏中过表达人β2肾上腺素能受体产生的心脏刺激和心脏抑制作用：β1肾上腺素能受体的区域差异和功能作用
Naunyn Schmiedebergs Arch Pharmacol. 2003 Apr;367(4):380-90. doi: 10.1007/s00210-002-0681-4. Epub 2003 Mar 4.
5
Atrial chronotropic reactivity to catecholamines in neonatal rats: Contribution of β-adrenoceptor subtypes.新生大鼠心房对儿茶酚胺的变时反应性：β-肾上腺素能受体亚型的作用
Eur J Pharmacol. 2015 Oct 5;764:385-394. doi: 10.1016/j.ejphar.2015.07.031. Epub 2015 Jul 14.
6
Disruption of inhibitory G-proteins mediates a reduction in atrial beta-adrenergic signaling by enhancing eNOS expression.抑制性G蛋白的破坏通过增强内皮型一氧化氮合酶（eNOS）的表达介导心房β-肾上腺素能信号传导的减少。
Cardiovasc Res. 2005 Sep 1;67(4):613-23. doi: 10.1016/j.cardiores.2005.04.034.
7
Decreased cardiac pacemaking and attenuated β-adrenergic response in TRIC-A knockout mice.TRIC-A 敲除小鼠的心脏起搏活动减少和β-肾上腺素能反应减弱。
PLoS One. 2020 Dec 21;15(12):e0244254. doi: 10.1371/journal.pone.0244254. eCollection 2020.
8
Age-related changes in cardiac electrophysiology and calcium handling in response to sympathetic nerve stimulation.与年龄相关的心脏电生理学和钙处理变化对交感神经刺激的反应。
J Physiol. 2018 Sep;596(17):3977-3991. doi: 10.1113/JP276396. Epub 2018 Aug 3.
9
Coupling of beta2-adrenoceptor to Gi proteins and its physiological relevance in murine cardiac myocytes.β2肾上腺素能受体与Gi蛋白的偶联及其在小鼠心肌细胞中的生理意义。
Circ Res. 1999;84(1):43-52. doi: 10.1161/01.res.84.1.43.
10
The influence of the adrenergic drugs on the electrical responses of the rabbit isolated heart atrium.肾上腺素能药物对家兔离体心房电反应的影响。
Physiol Bohemoslov. 1970;19(1):19-22.

本文引用的文献

1
Deficiency of PKD2L1 (TRPP3) Exacerbates Pathological Cardiac Hypertrophy by Augmenting NCX1-Mediated Mitochondrial Calcium Overload.PKD2L1（TRPP3）缺乏通过增强 NCX1 介导的线粒体钙超载加剧病理性心肌肥厚。
Cell Rep. 2018 Aug 7;24(6):1639-1652. doi: 10.1016/j.celrep.2018.07.022.
2
Disruption of polycystin-L causes hippocampal and thalamocortical hyperexcitability.多囊蛋白-L的破坏会导致海马体和丘脑皮质兴奋性过高。
Hum Mol Genet. 2016 Feb 1;25(3):448-58. doi: 10.1093/hmg/ddv484. Epub 2015 Nov 26.
3
Acid-induced off-response of PKD2L1 channel in Xenopus oocytes and its regulation by Ca(2.).
非洲爪蟾卵母细胞中PKD2L1通道的酸诱导关闭反应及其钙（2+）调节
Sci Rep. 2015 Oct 27;5:15752. doi: 10.1038/srep15752.
4
Effects of propofol on electrocardiogram measures in mice.丙泊酚对小鼠心电图指标的影响。
J Pharmacol Sci. 2014;126(4):351-8. doi: 10.1254/jphs.14181FP. Epub 2014 Nov 19.
5
Cardiovascular complications in autosomal dominant polycystic kidney disease.常染色体显性多囊肾病中的心血管并发症
Curr Hypertens Rev. 2013 Feb;9(1):2-11. doi: 10.2174/1573402111309010002.
6
Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases.多囊蛋白-2 和磷酸二酯酶 4C 是纤毛 A 激酶锚定蛋白复合物的组成部分，该复合物在囊性肾病中被破坏。
Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10679-84. doi: 10.1073/pnas.1016214108. Epub 2011 Jun 13.
7
Interaction between PKD1L3 and PKD2L1 through their transmembrane domains is required for localization of PKD2L1 at taste pores in taste cells of circumvallate and foliate papillae.PKD1L3 和 PKD2L1 通过它们的跨膜结构域相互作用，对于 PKD2L1 在环状和叶状乳头味细胞的味孔中的定位是必需的。
FASEB J. 2010 Oct;24(10):4058-67. doi: 10.1096/fj.10-162925. Epub 2010 Jun 10.
8
Modified sympathetic nerve system activity with overexpression of the voltage-dependent calcium channel beta3 subunit.电压依赖性钙通道β3亚基过表达导致的交感神经系统活动改变。
J Biol Chem. 2008 Sep 5;283(36):24554-60. doi: 10.1074/jbc.M802319200. Epub 2008 Jul 15.
9
TRP channel and cardiovascular disease.瞬时受体电位通道与心血管疾病。
Pharmacol Ther. 2008 Jun;118(3):337-51. doi: 10.1016/j.pharmthera.2008.03.008. Epub 2008 Apr 10.
10
Direct binding of alpha-actinin enhances TRPP3 channel activity.α-辅肌动蛋白的直接结合增强TRPP3通道活性。
J Neurochem. 2007 Dec;103(6):2391-400. doi: 10.1111/j.1471-4159.2007.04940.x. Epub 2007 Oct 17.