Department of Pharmaceutical Pathophysiology, Faculty of Pharmacy, Medical University of Gdansk, Dębinki 7 Street, 80-211 Gdansk, Poland.
Poznan University of Physical Education, Królowej Jadwigi 27/39 Street, 61-871 Poznan, Poland.
Int J Mol Sci. 2022 Jan 11;23(2):748. doi: 10.3390/ijms23020748.
This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats' blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient's health due to the common use of glucocorticoids like Dex.
这项研究调查了地塞米松（Dex）治疗对长时间游泳后血液和骨骼肌代谢物水平以及与能量代谢相关的骨骼肌酶活性的影响。为了评估 Dex 治疗、游泳以及这些因素结合是否对分析数据起作用，将大鼠随机分为四组：盐水处理不运动和运动以及 Dex 处理不运动和运动。两组运动组的动物都进行了长时间游泳。根据标准比色法和荧光法测量骨骼肌和血液中的脂质代谢物、葡萄糖和乳酸浓度。此外，还测量了骨骼肌中与有氧和无氧代谢相关的酶的活性。结果表明，Dex 治疗导致大鼠体重减轻，并增加了血液中的脂质代谢物，但并未改变骨骼肌中的这些变化。有趣的是，在 Dex 治疗 9 天后进行的长时间游泳显著加剧了 Dex 诱导的变化；然而，骨骼肌酶活性没有差异。这项研究首次显示了运动和 Dex 对脂质代谢某些元素的累积效应，由于像 Dex 这样的糖皮质激素的普遍使用，这对患者的健康似乎至关重要。
