Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
Guangxi Medical University, No.22 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
BMC Immunol. 2022 Jan 22;23(1):3. doi: 10.1186/s12865-022-00476-6.
This study was aimed to identify the biomarkers for diagnosis and reveal the immune microenvironment changes in ankylosing spondylitis (AS).
GSE73754 was downloaded for the co-expression network construction and immune cell analyses. Flow cytometric analysis was performed to validate the results of bioinformatics analysis. Gene set enrichment analysis (GSEA) was performed to investigate the potential biological characteristic between different phenotypes. Pearson correlation analysis between the hub genes and the xCell score of immune cell types was performed.
Signal transducer and activator of transcription 3 (STAT3) and Spi-1 proto-oncogene (SPI1) was identified as the hub genes in the datasets GSE73754. And the t-test showed that the expression level of STAT3 and SPI1 in the GSE73754 was significantly higher in AS and human leukocyte antigen (HLA)-B27(+) groups. Flow cytometric analysis showed that natural killer T cells (NKT) cells were upregulated, while Th1 cells were down-regulated in AS, which was consistent with the results obtained from bioinformatics analysis. STAT3 and SPI1 was correlated with the NKT cells and Th1 cells.
STAT3 and SPI1 may be a key cytokine receptor in disease progression in AS.
本研究旨在鉴定强直性脊柱炎（AS）的诊断生物标志物，并揭示其免疫微环境变化。
下载 GSE73754 进行共表达网络构建和免疫细胞分析。流式细胞术分析用于验证生物信息学分析的结果。基因集富集分析（GSEA）用于研究不同表型之间的潜在生物学特征。对关键基因与免疫细胞类型的 xCell 评分之间进行 Pearson 相关性分析。
信号转导和转录激活因子 3（STAT3）和 Sp1 原癌基因（SPI1）被确定为数据集 GSE73754 中的关键基因。t 检验显示，在 GSE73754 中，STAT3 和 SPI1 的表达水平在 AS 和人类白细胞抗原（HLA）-B27（+）组中显著升高。流式细胞术分析显示，AS 中自然杀伤 T 细胞（NKT）细胞上调，而 Th1 细胞下调，这与生物信息学分析的结果一致。STAT3 和 SPI1 与 NKT 细胞和 Th1 细胞相关。
STAT3 和 SPI1 可能是 AS 疾病进展中的关键细胞因子受体。
