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野生型和l(3)pl(致死-多倍体)海德氏果蝇雄性生殖细胞中微管结构的间接免疫荧光显微镜观察。

Indirect immunofluorescence microscopy of microtubular structures in male germ cells of wildtype and l(3)pl (lethal-polyploid) Drosophila hydei.

作者信息

Rungger-Brändle E, Franke W W, Osborn M, Weber K

出版信息

Differentiation. 1978 May 26;10(3):187-91. doi: 10.1111/j.1432-0436.1978.tb00963.x.

DOI:10.1111/j.1432-0436.1978.tb00963.x
PMID:350682
Abstract

Tubulin-containing structures of the male germ cells of Drosophila hydei crossreact in indirect immunofluorescence microscopy with antibody directed against homogeneous porcine brain tubulin. There is no detectable difference in reactivity between germ cells of wildtype flies and the mutant l(3)pl (lethal-polyploid) which is characterized by microtubular abnormalities. However, the technique of indirect immunofluorescence microscopy allows the direct visualization of several abnormalities in the arrangement of the microtubular system of the mutant, particularly in the axonemal complex.

摘要

海德果蝇雄性生殖细胞中含微管蛋白的结构,在间接免疫荧光显微镜下与针对猪脑匀质微管蛋白的抗体发生交叉反应。野生型果蝇的生殖细胞与以微管异常为特征的突变体l(3)pl(致死-多倍体)的生殖细胞之间,在反应性上没有可检测到的差异。然而,间接免疫荧光显微镜技术能直接观察到突变体微管系统排列中的几种异常情况,特别是在轴丝复合体中。

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Indirect immunofluorescence microscopy of microtubular structures in male germ cells of wildtype and l(3)pl (lethal-polyploid) Drosophila hydei.野生型和l(3)pl(致死-多倍体)海德氏果蝇雄性生殖细胞中微管结构的间接免疫荧光显微镜观察。
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