使用基质固定化重组质粒纯化链特异性兔珠蛋白互补DNA。

Use of matrix-immobilised recombinant plasmids to purify chain-specific rabbit globin complementary DNAs.

作者信息

Coutelle C, Ioannou P, Williamson R

出版信息

Gene. 1978 Apr;3(2):113-22. doi: 10.1016/0378-1119(78)90055-0.

DOI:10.1016/0378-1119(78)90055-0

Abstract

The cloning of DNA sequences in plasmid recombinants has made it possible to amplify specific sequences to an extent that they can be used for preparative purposes. We describe the use of rabbit globin DNA sequences cloned in the plasmid pCR1 and covalently bound to Sepharose 4B for the purification of chain-specific rabbit alpha- and beta-globin cDNAs. These purified probes were then used to estimate the length of the alpha- and beta-globin DNA sequences inserted into the recombinant plasmid. The technique should allow the rapid isolation of sequence-specific cDNA, RNA and genomic DNA.

摘要

将DNA序列克隆到质粒重组体中，使得扩增特定序列成为可能，扩增程度足以将其用于制备目的。我们描述了使用克隆于质粒pCR1并共价结合到琼脂糖凝胶4B上的兔珠蛋白DNA序列来纯化链特异性兔α和β珠蛋白cDNA。然后使用这些纯化的探针来估计插入重组质粒中的α和β珠蛋白DNA序列的长度。该技术应能快速分离序列特异性cDNA、RNA和基因组DNA。

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