体外合成的α和β小鼠珠蛋白基因序列的克隆与扩增。

Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.

作者信息

Rougeon F, Mach B

出版信息

Gene. 1977 May;1(3-4):229-39. doi: 10.1016/0378-1119(77)90047-6.

Abstract

New chimeric Escherichia coli plasmids containing alpha or beta globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described.

摘要

构建了含有小鼠α或β珠蛋白基因序列的新型嵌合大肠杆菌质粒。从小鼠珠蛋白mRNA通过两步体外反应合成的双链DNA，分别用dG和dC对两种DNA进行加尾后，插入大肠杆菌质粒pCR1。所描述的一些小鼠珠蛋白质粒含有至少90%的珠蛋白mRNA序列，因此包含珠蛋白基因的完整翻译序列。还描述了这些重组质粒的一些可能用途。