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Gas chromatography-mass spectrometry analysis of tert.-butyldimethylsilyl derivatives of 2-acetylaminofluorene and metabolites in isolated rat hepatocytes.

作者信息

Diez Ibañez M A, Chessebeuf-Padieu M, Nordmann P, Padieu P

机构信息

Laboratoire de Biochimie Médicale, Faculté de Médecine, Dijon, France.

出版信息

Cell Biol Toxicol. 1987 Sep;3(3):327-40. doi: 10.1007/BF00117869.

DOI:10.1007/BF00117869
PMID:3507261
Abstract

A new technique for the conversion of 2-acetylaminofluorene and several ring-hydroxylated metabolites to mono- and di-tert.-butyldimethylsilyl derivatives was developed to permit their analysis by gas chromatography-mass spectrometry in order to quantify the metabolism of 2-acetylaminofluorene incubated in freshly isolated rat hepatocytes. This new gas chromatography-mass spectrometry method allowed the separation, identification and quantitation of seven known metabolites comprising five arylhydroxylated compounds, 2-aminofluorene and N-hydroxy-2-acetylaminofluorene.

摘要

相似文献

1
Gas chromatography-mass spectrometry analysis of tert.-butyldimethylsilyl derivatives of 2-acetylaminofluorene and metabolites in isolated rat hepatocytes.
Cell Biol Toxicol. 1987 Sep;3(3):327-40. doi: 10.1007/BF00117869.
2
[Biosynthesis of metabolites of 2-acetylaminofluorene by isolated hepatocytes of rats: study by gas chromatography-mass spectrometry].[大鼠离体肝细胞对2-乙酰氨基芴代谢产物的生物合成：气相色谱-质谱联用研究]
C R Seances Soc Biol Fil. 1986;180(4):434-40.
3
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J Chromatogr. 1989 Dec 8;483:21-32. doi: 10.1016/s0021-9673(01)93107-3.
4
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5
High-performance liquid chromatographic analysis of 2-aminofluorene and its metabolites in monolayer cultures of rat hepatocytes.大鼠肝细胞单层培养物中2-氨基芴及其代谢产物的高效液相色谱分析
Anal Biochem. 1986 Feb 15;153(1):39-44. doi: 10.1016/0003-2697(86)90057-6.
6
Gas-liquid chromatography and mass spectral analysis of mono-, di- and tricarboxylates as their tert.-butyldimethylsilyl derivatives.作为叔丁基二甲基硅烷基衍生物的单羧酸盐、二羧酸盐和三羧酸盐的气-液色谱和质谱分析。
J Chromatogr. 1986 Jun 27;361:117-30. doi: 10.1016/s0021-9673(01)86899-0.
7
Preparation of enol-tert.-butyldimethylsilyl and mixed tert.-butyldimethylsilyl-trimethylsilyl ethers for gas chromatography--mass spectrometry of steroids and bile acids.
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Capillary gas chromatography-mass spectrometry of valproic acid metabolites in serum and urine using tert.-butyldimethylsilyl derivatives.
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引用本文的文献

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Toxic effects of 7 beta-hydroxycholesterol on rat liver primary cultures, epithelial lines and co-cultures.
Cell Biol Toxicol. 1989 Nov;5(3):261-70. doi: 10.1007/BF01795355.
2
Maintenance of liver function in long term culture of hepatocytes following in vitro or in vivo Ha-rasEJ transfection.体外或体内Ha-rasEJ转染后肝细胞长期培养中肝功能的维持。
Cell Biol Toxicol. 1991 Oct;7(4):327-45. doi: 10.1007/BF00124069.

本文引用的文献

1
The N- and ring-hydroxylation of 2-acetylaminofluorene and the failure to detect N-acetylation of 2-aminofluorene in the dog.2-乙酰氨基芴的N-和环羟基化以及在犬体内未检测到2-氨基芴的N-乙酰化。
Cancer Res. 1963 Jun;23:790-800.
2
The N- and ringhydroxylation of 2-acetylaminofluorene during carcinogenesis in the rat.大鼠致癌过程中2-乙酰氨基芴的N-羟基化和环羟基化。
Cancer Res. 1960 Jul;20:950-62.
3
N-Hydroxylation: A new metabolic reaction observed in the rat with the carcinogen 2-acetylaminofluorene.N-羟基化：在大鼠体内观察到的与致癌物2-乙酰氨基芴相关的一种新的代谢反应。
J Biol Chem. 1960 Mar;235:885-8.
4
N-Hydroxy-2-acetylaminofluorene: a metabolite of 2-acetylaminofluorene with increased carcinogenic activity in the rat.N-羟基-2-乙酰氨基芴：2-乙酰氨基芴的一种代谢产物，在大鼠中具有增强的致癌活性。
Cancer Res. 1961 Jul;21:815-24.
5
Chromatographic separation of some metabolites of the carcinogen N-2-fluorenylacetamide.致癌物N-2-芴基乙酰胺某些代谢物的色谱分离
J Natl Cancer Inst. 1956 Sep;17(3):363-74.
6
Urinary metabolites of the carcinogen N-2-fluorenylacetamide.致癌物N-2-芴基乙酰胺的尿液代谢物。
J Natl Cancer Inst. 1956 Sep;17(3):345-61.
7
Catalysis of divergent pathways of 2-acetylaminofluorene metabolism by multiple forms of cytochrome P-450.多种形式的细胞色素P-450催化2-乙酰氨基芴代谢的不同途径。
Cancer Res. 1980 Dec;40(12):4456-9.
8
N-Substituted aryl compounds in carcinogenesis and mutagenesis.致癌作用和诱变作用中的N-取代芳基化合物。
Natl Cancer Inst Monogr. 1981 Dec(58):1-7.
9
An improved high-pressure liquid chromatographic assay for 2-acetylaminofluorene and eight of its metabolites.
Anal Biochem. 1981 May 1;113(1):62-7. doi: 10.1016/0003-2697(81)90044-0.
10
Metabolism of 2-acetylaminofluorene in primary rat hepatocyte cultures.2-乙酰氨基芴在原代大鼠肝细胞培养物中的代谢。
J Toxicol Environ Health. 1981 Jan;7(1):93-106. doi: 10.1080/15287398109529961.