Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, PR People's Republic of China.
Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety, College of Animal Science and Technology, Anhui Agricultural University, Hefei, PR People's Republic of China.
Avian Pathol. 2022 Apr;51(2):154-163. doi: 10.1080/03079457.2022.2031881. Epub 2022 Feb 28.
T6SS (type VI secretion system) is a type of nano-syringe that exists in APEC (avian pathogenic ). Hcp (haemolysin-coregulated protein) of T6SS participates in the regulation of virulence during APEC infection. However, whether plays a role in bacterial colonization by expression in host cells remains unclear. In this study, we analysed the biological characteristics of the mutant strain. Our results showed that the gene was involved in the regulation of bacterial motility, biofilm formation, anti-serum and anti-oxidative stress. Moreover, our data indicate that the colonization of the mutation strain (Δ) in the lung, liver and kidney of chickens decreased significantly. Hence, overexpression of Hcp2b protein in DF-1 cells was used to analyse the effect of Hcp2b on colonization of APEC. Proteomics analysis showed that overexpression of Hcp2b induced differentially expressed proteins in DF-1 cells (230 were significantly upregulated and 96 were significantly downregulated) and differentially expressed proteins were enriched in keratin filament. In conclusion, our data indicated that promoted the colonization of APEC by affecting the expression of keratin filament.
T6SS（Type VI Secretion System）是一种存在于 APEC（禽致病性）中的纳米注射器。T6SS 的 Hcp（溶血素调节蛋白）参与 APEC 感染期间的毒力调节。然而，在宿主细胞中表达是否通过表达在宿主细胞中发挥作用，其在细菌定植中的作用尚不清楚。在这项研究中，我们分析了突变株的生物学特性。结果表明，基因参与细菌运动性、生物膜形成、抗血清和抗氧化应激的调节。此外，我们的数据表明，鸡肺、肝和肾中突变株（Δ）的定植明显减少。因此，过表达 DF-1 细胞中的 Hcp2b 蛋白用于分析 Hcp2b 对 APEC 定植的影响。蛋白质组学分析表明，Hcp2b 的过表达诱导 DF-1 细胞中差异表达蛋白（230 个显著上调，96 个显著下调），差异表达蛋白在角蛋白丝中富集。总之，我们的数据表明，通过影响角蛋白丝的表达，促进了 APEC 的定植。
