利用鲎试剂检测法快速检测实验性大肠杆菌眼内炎

Rapid detection of experimental E. coli endophthalmitis by the Limulus lysate test.

作者信息

Avallone A N, Parrett C, Smith R E, Meyers R, Chitjian P A

出版信息

Invest Ophthalmol Vis Sci. 1978 Jun;17(6):528-32.

Abstract

Experimental E. coli endophthalmitis was produced in rabbits. The Limulus lysate test was applied to aqueous and vitreous samples at various intervals after the intravitreal injection of E. coli organisms. Results indicated that this test is feasible using vitreous and aqueous samples. The Limulus test was positive for E. coli endotoxin within hours after infection, requiring only 1 hr to determine the presence of endotoxin after sampling. This test may have some value in the rapid diagnosis of gram-negative endophthalmitis.

摘要

在兔子身上制造了实验性大肠杆菌眼内炎。在玻璃体内注射大肠杆菌后，于不同时间间隔对房水和玻璃体样本进行鲎试剂检测。结果表明，使用玻璃体和房水样本进行该检测是可行的。感染后数小时内，鲎试剂检测对大肠杆菌内毒素呈阳性反应，采样后仅需1小时即可确定内毒素的存在。该检测在革兰氏阴性菌眼内炎的快速诊断中可能具有一定价值。