产朊假丝酵母（球拟酵母）亮氨酸转运RNA 1的核苷酸序列。

Nucleotide sequence of leucine transfer RNA 1 from Candida (Torulopsis) utilis.

作者信息

Murasugi A, Takemura S

出版信息

J Biochem. 1978 Apr;83(4):1029-38. doi: 10.1093/oxfordjournals.jbchem.a131991.

DOI:10.1093/oxfordjournals.jbchem.a131991

Abstract

Two species of 32P-labelled leucine tRNA were highly purified from Candida (Torulopsis) utilis by successive column chromatographies. The purified major species of leucine tRNA 1 was completely digested with ribonuclease T1 [EC 3.1.4.8] and with pancreatic ribonuclease A [EC 3.1.4.22]. The resulting fragments were fractionated, and their nucleotide sequences were determined according to Barrell (1). The results of analyses of the two ribonuclease digests were consistent with each other, and indicated that this tRNA is composed of 85 nucleotide residues, including 14 modified nucleotides. A tentative total sequence has been derived on the basis of several features in the cloverleaf structure for tRNA.

摘要

通过连续柱色谱法从产朊假丝酵母（球拟酵母）中高度纯化出两种32P标记的亮氨酸tRNA。纯化后的主要亮氨酸tRNA 1物种被核糖核酸酶T1 [EC 3.1.4.8]和胰核糖核酸酶A [EC 3.1.4.22]完全消化。对所得片段进行分级分离，并根据巴雷尔（1）的方法测定其核苷酸序列。两种核糖核酸酶消化产物的分析结果相互一致，表明该tRNA由85个核苷酸残基组成，包括14个修饰核苷酸。基于tRNA三叶草结构的几个特征得出了一个初步的完整序列。

相似文献

1

Nucleotide sequence of leucine transfer RNA 1 from Candida (Torulopsis) utilis.产朊假丝酵母（球拟酵母）亮氨酸转运RNA 1的核苷酸序列。

J Biochem. 1978 Apr;83(4):1029-38. doi: 10.1093/oxfordjournals.jbchem.a131991.

2

The primary structure of cytoplasmic initiator transfer ribonucleic acid from Torulopsis utilis.

J Biochem. 1979 Aug;86(2):335-46. doi: 10.1093/oxfordjournals.jbchem.a132531.

3

The primary structure of a non-initiating methionine-specific tRNA from brewer's yeast.来自酿酒酵母的一种非起始甲硫氨酸特异性tRNA的一级结构。

Eur J Biochem. 1976 Sep;68(1):209-17. doi: 10.1111/j.1432-1033.1976.tb10780.x.

4

The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. I. Purification and complete digestion with ribonuclease T1 and pancreatic ribonuclease A.来自面包酵母的非起始甲硫氨酸转移核糖核酸的一级结构。I. 用核糖核酸酶T1和胰核糖核酸酶A进行纯化及完全消化。

J Biochem. 1976 Nov;80(5):937-50. doi: 10.1093/oxfordjournals.jbchem.a131381.

5

The primary structure of alanine transfer ribonucleic acid 1 from Torulopsis utilis. I. Complete digestion with pancreatic ribonuclease and ribonuclease T1.

J Biochem. 1973 Aug;74(2):313-22.

6

The primary structure of alanine transfer ribonucleic acid 1 from Torulopsis utilis. II. Partial digestion with ribonuclease T1 and derivation of the complete sequence.

J Biochem. 1973 Aug;74(2):323-33.

7

Yeast tRNA Leu UAG. Purification, properties and determination of the nucleotide sequence by radioactive derivative methods.酵母tRNA亮氨酸UAG。通过放射性衍生物方法进行纯化、性质研究及核苷酸序列测定。

Eur J Biochem. 1979 Jan 2;93(1):79-94. doi: 10.1111/j.1432-1033.1979.tb12797.x.

8

Nucleotide sequence of 5 S RNA from Torulopsis utilis.产朊假丝酵母5S RNA的核苷酸序列

FEBS Lett. 1974 Mar 15;40(1):106-9. doi: 10.1016/0014-5793(74)80904-x.

9

Physico-chemical studies on conformation of a complex reconstituted from half molecules of Torulopsis utilis tyrosine transfer ribonucleic acid.

J Biochem. 1972 Nov;72(5):1185-95. doi: 10.1093/oxfordjournals.jbchem.a130006.

10

Identification and assignment of base pairs in three helical stems of Torulopsis utilis ribosomal 5S RNA and its RNase T1 and RNase T2 cleaved fragments via 500-MHz proton homonuclear overhauser enhancements.通过500兆赫质子同核Overhauser增强效应鉴定产朊假丝酵母核糖体5S RNA及其核糖核酸酶T1和核糖核酸酶T2切割片段的三个螺旋茎中的碱基对并进行归属。

Biochemistry. 1986 Sep 9;25(18):5117-25. doi: 10.1021/bi00366a021.

引用本文的文献

1

Effects of anticodon 2'-O-methylations on tRNA codon recognition in an Escherichia coli cell-free translation.反密码子2'-O-甲基化对大肠杆菌无细胞翻译中tRNA密码子识别的影响

RNA. 2000 May;6(5):680-6. doi: 10.1017/s1355838200000029.

2

Compilation of tRNA sequences and sequences of tRNA genes.tRNA序列及tRNA基因序列的汇编。

Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2127-71. doi: 10.1093/nar/19.suppl.2127.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。