Gruhl H, Feldmann H
Eur J Biochem. 1976 Sep;68(1):209-17. doi: 10.1111/j.1432-1033.1976.tb10780.x.
tRNA3Met, one of the non-initiating methionine-specific tRNAs in brewer's yeast was purified from bulk tRNA labelled with [32P]phosphate by two column chromatographic steps. The primary structure of this tRNA was determined by the usual fingerprinting technique. Analyses of the isolated nucleotides and oligonucleotides from digests with pancreatic and T1 ribonucleases were in good agreement and stated that tRNA3Met consists of 76 nucleotide residues including 13 minor nucleotides. Overlaps from which the complete sequence could be deduced were derived from the analyses of 15 fragments obtained by partial digestion with T1 ribonuclease.
tRNA3Met是啤酒酵母中一种非起始甲硫氨酸特异性tRNA，通过两步柱色谱法从用[32P]磷酸盐标记的大量tRNA中纯化得到。该tRNA的一级结构通过常规指纹技术确定。对用胰核糖核酸酶和T1核糖核酸酶消化得到的分离核苷酸和寡核苷酸的分析结果吻合良好，表明tRNA3Met由76个核苷酸残基组成，包括13个稀有核苷酸。通过对T1核糖核酸酶部分消化得到的15个片段的分析得出了可推导完整序列的重叠部分。
