Nakagawa Y, Takayama K, Ueda H, Machida Y, Nagai T
Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
Drug Des Deliv. 1987 Dec;2(2):99-107.
Bovine serum albumin nanospheres (BSA-NS) of mean diameter about 170 nm were prepared by means of the tanning method with glutaraldehyde, and their efficacy as drug targeting carriers was evaluated. To gain insight of biodegradability, BSA microspheres (BSA-MS) were first administered to rats and their distributions in the lungs and liver were observed by a scanning electron microscope. A large amount of BSA-MS was found in the lungs and their surface was slightly degraded at 1 week after the administration. For investigating biocompatibility, the weight increase of the spleen and liver was measured after the administration of the BSA-NS to mice. The spleen weight of the group receiving BSA-NS was equivalent to that of the control group, though the liver weight was significantly increased. It was observed that conjugates of BSA-NS with antibody selectively concentrated on the surface of Sepharose beads which were coated with antigen.
采用戊二醛鞣酸法制备了平均直径约为170 nm的牛血清白蛋白纳米球（BSA-NS），并评估了其作为药物靶向载体的功效。为了解其生物降解性，首先将牛血清白蛋白微球（BSA-MS）给予大鼠，并通过扫描电子显微镜观察其在肺和肝中的分布。给药1周后，在肺中发现大量BSA-MS，其表面略有降解。为研究生物相容性，将BSA-NS给予小鼠后，测量脾脏和肝脏的重量增加。接受BSA-NS的组的脾脏重量与对照组相当，尽管肝脏重量显著增加。观察到BSA-NS与抗体的缀合物选择性地浓缩在包被有抗原的琼脂糖珠表面。
