鼠气管整体血管和淋巴管成像。
Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.
机构信息
Department of Anatomy, Cardiovascular Research Institute, University of California, San Francisco, CA, USA.
UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2022;2441:115-134. doi: 10.1007/978-1-0716-2059-5_10.
Changes in blood vessels and lymphatics in health and disease are easier to understand and interpret when studied microscopically in three dimensions. The mouse trachea is a simple, yet powerful, and versatile model system in which to achieve this. We describe practical immunohistochemical methods for fluorescence and confocal microscopy of wholemounts of the mouse trachea to achieve this purpose in which the entire vasculature can be visualized from the organ level to the cellular and subcellular level. Blood vessels and lymphatics have highly stereotyped vascular architectures that repeat in arcades between the tracheal cartilages. Arterioles, capillaries, and venules can be easily identified for the blood vessels, while the lymphatics consist of initial lymphatics and collecting lymphatics. Even small abnormalities in either blood vessels or lymphatics can be noticed and evaluated in three dimensions. We and others have used the mouse trachea for examining in situ angiogenesis and lymphangiogenesis, vascular development and regression, vessel patency, differences in transgenic mice, and pathological changes, such as increased vascular permeability induced by inflammatory mediators.
在健康和疾病状态下，血管和淋巴管的变化，如果在显微镜下从三维角度进行研究，将更容易理解和解释。小鼠气管是一种简单但功能强大且用途广泛的模型系统，可实现这一目标。我们描述了用于荧光和共聚焦显微镜的整个小鼠气管的免疫组织化学方法，以达到此目的，其中可以从器官水平到细胞和亚细胞水平可视化整个脉管系统。血管和淋巴管具有高度刻板的血管结构，在气管软骨之间的拱廊中重复。小动脉、毛细血管和小静脉可以很容易地识别出血管，而淋巴管由初始淋巴管和收集淋巴管组成。即使是血管或淋巴管的微小异常也可以在三维空间中注意到并进行评估。我们和其他人已经使用小鼠气管来检查原位血管生成和淋巴管生成、血管发育和退化、血管通畅性、转基因小鼠的差异以及病理变化，例如炎症介质引起的血管通透性增加。
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