Renewable Materials Program, Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, Idaho 83844-1132, United States.
Plant Physiology, Department of Horticulture, Agricultural Science Center North, University of Kentucky, Lexington, Kentucky 40546, United States.
J Agric Food Chem. 2022 Feb 9;70(5):1689-1703. doi: 10.1021/acs.jafc.1c07176. Epub 2022 Jan 31.
The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) H-C nuclear magnetic resonance (NMR). The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons. In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively. The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N. The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes. Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected. The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin. These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
采用高效液相色谱法（HPLC）、热裂解-气相色谱-质谱法（Py-GCMS）、X 射线衍射（XRD）和二维（2D）H-C 核磁共振（NMR）对两种甜高粱品种（Della（D）及其变种 RG）的细胞壁组成（木质素和多糖）在节间（IN）和节点（N）进行了评估。茎秆分别于 2018 年（D1 和 RG1）和 2019 年（D2 和 RG2）生长。在 RG1 中，IN 和 N 中的 Klason 木质素分别减少了 16-44%和 2-26%。分析还表明，高粱茎秆中的木质素富含愈创木基单元，且 RG1 和 RG2 中的丁香基/愈创木基比分别增加了 96%和 2 倍以上，在 IN 中增加了 61%，在 N 中增加了 23%。RG1 中 IN 的葡聚糖含量减少了 23-27%，RG2 中减少了 17-22%。由于纤维素和半纤维素糖基的变化，检测到结构发生了变化。非酰化和γ-酰化的β-O-4 键是木质素中主要的单元间键。这些结果表明，由于 RG 的变化，茎秆的组成发生了变化，生长季节可能会影响它们的机械和倒伏行为。
