聚酰胺-胺树枝状大分子 - G3 修饰 TiO2 对水溶液中金黄色葡萄球菌和大肠杆菌的抗菌作用。

Antibacterial effect of TiO modified with poly-amidoamine dendrimer - G3 on S. aureus and E. coli in aqueous solutions.

机构信息

Department of Environmental Health Engineering, School of public Health, Ardabil University of Medical Sciences, Ardabil, Iran E-mail:

Department of Environmental Health Engineering, Khalkhal University of Medical Sciences, Khalkhal, Iran.

出版信息

Water Sci Technol. 2022 Jan;85(2):605-616. doi: 10.2166/wst.2022.007.

DOI:10.2166/wst.2022.007

PMID:35100142

Abstract

This study investigated bacterial removal using TiO nanoparticles (NPs) modified with poly-amidoamine dendrimer macromolecule (PAMAM, G3). The PAMAM G3/TiO (nanohybrid) was used to specify antibacterial properties via broth microdilution (MBC-Minimum Bactericidal Concentration and MIC-Minimum Inhibitory Concentration-determination), paper disc diffusion, and surface plate count methods. The nanohybrid was characterized via the different techniques. The effects of different factors including initial bacteria count, run time, solution pH, and the nanohybrid concentration were studied. The nanohybrid cytotoxicity was studied on AGS and MKN45 cells line by MTT assay. It was revealed that the nanohybrid was effective in intercepting both bacterial strains growth. The MIC value for S. aureus and E. coli were determined to be 4 and 2 μg/mL, respectively. The MBC value for both strains were calculated to be 32 μg/mL. The results showed removal efficiency of 100% for S. aureus and E. coli bacteria in optimum situation. The decrease in cell viability in the dosage of 32 μg/mL after 72 h treatment for AGS and MKN45 cells line were shown to be 6.2 and 4.6%, respectively. The nanohybrid was able to decrease the S. aureus and E. coli count in solution, which meets the drinking water criterions aligned with WHO guidelines.

摘要

本研究利用聚酰胺-胺树枝状大分子（PAMAM，G3）修饰的 TiO2 纳米粒子（NPs）研究了细菌的去除。通过肉汤微量稀释法（MBC-最小杀菌浓度和 MIC-最小抑菌浓度测定）、纸片扩散法和表面平板计数法，使用 PAMAM G3/TiO2（纳米复合材料）来确定抗菌性能。通过不同的技术对纳米复合材料进行了表征。研究了初始细菌数、运行时间、溶液 pH 值和纳米复合材料浓度等不同因素的影响。通过 MTT 测定法研究了纳米复合材料对 AGS 和 MKN45 细胞系的细胞毒性。结果表明，纳米复合材料有效地阻止了两种细菌的生长。金黄色葡萄球菌和大肠杆菌的 MIC 值分别确定为 4 和 2 μg/mL。两种菌株的 MBC 值均计算为 32 μg/mL。结果表明，在最佳条件下，金黄色葡萄球菌和大肠杆菌的去除效率达到 100%。AGS 和 MKN45 细胞系在 32 μg/mL 剂量下处理 72 h 后，细胞活力分别下降 6.2%和 4.6%。纳米复合材料能够降低溶液中金黄色葡萄球菌和大肠杆菌的数量，符合世界卫生组织（WHO）指南规定的饮用水标准。