Divyashree M, Mani Madhu K, Karunasagar Indrani
Department of Biomedical Sciences, Nitte University Center for Science Education and Research (NUCSER), NITTE (Deemed to be University), Paneer Campus, Kotekar-Beeri Road, Deralakatte, Mangalore 575018, India E-mail:
DST-TEC, NITTE (DU), Paneer Campus, Kotekar-Beeri Road, Deralakatte, Mangaluru 575018, Karnataka, India.
J Water Health. 2022 Jan;20(1):176-184. doi: 10.2166/wh.2021.223.
The study aimed to examine the relationship between antibiotic resistance, biofilm formation and genes responsible for biofilm formation. Sixty-six Pseudomonas aeruginosa isolates were obtained from hospital wastewater and analyzed for their antibiotic resistance. Biofilm production among the isolates was tested by indirect quantification method crystal violet assay. Biofilm-associated genes among these isolates psl, alg, and pel were also checked. The maximum resistance was observed for ampicillins (88.24%) followed by nalidixic (83.82%), and nitrofurantoin (64.71%), respectively. Biofilm phenotypes are distributed in the following categories: high 39.39% (n = 26); moderate 57.57% (n = 38), and weak 3.0% (n = 2). Among the total isolates, biofilm-associated genes were detected in 84.84% (n = 56) of isolates and the remaining isolates 15.15% (n = 10) did not harbor any genes. In this study, pslB was the most predominant gene observed (71.21%, n = 47) followed by pslA (57.57%, n = 38), pelA (45.45%, n = 30), algD (43.93%, n = 29), and pelD (27.27%, n = 18), respectively. The present study reveals that the majority of the isolates are multidrug resistant being moderate and high biofilm formers. The study implies that biofilm acts as a machinery for bacteria to survive in the hospital effluent which is an antibiotic stress environment.
该研究旨在探讨抗生素耐药性、生物膜形成与生物膜形成相关基因之间的关系。从医院废水中获取了66株铜绿假单胞菌分离株，并对其抗生素耐药性进行了分析。采用间接定量方法结晶紫测定法检测分离株中的生物膜产生情况。还检查了这些分离株中与生物膜相关的基因psl、alg和pel。观察到对氨苄西林的耐药率最高（88.24%），其次是萘啶酸（83.82%）和呋喃妥因（64.71%）。生物膜表型分布在以下类别：高39.39%（n = 26）；中度57.57%（n = 38），弱3.0%（n = 2）。在所有分离株中，84.84%（n = 56）的分离株检测到与生物膜相关的基因，其余15.15%（n = 10）的分离株未携带任何基因。在本研究中，观察到pslB是最主要的基因（71.21%，n = 47），其次是pslA（57.57%，n = 38）、pelA（45.45%，n = 30）、algD（43.93%，n = 29）和pelD（27.27%，n = 18）。本研究表明，大多数分离株具有多重耐药性，是中度和高度生物膜形成者。该研究表明，生物膜是细菌在医院废水这种抗生素应激环境中生存的一种机制。
