Faculty of Medicine, Department of Parasitology, University of Ordu, Ordu, Turkey.
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, University of Ordu, Ordu, Turkey E-mail:
J Water Health. 2022 Jan;20(1):193-204. doi: 10.2166/wh.2021.250.
Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: KJ094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations.
棘阿米巴属感染可导致肉芽肿性阿米巴脑炎和棘阿米巴角膜炎。水是棘阿米巴属物种的重要栖息地，因此，研究这种自由生活的阿米巴在水源中的存在有助于了解感染动态。本研究旨在调查和报告黑海中奥尔杜和吉雷松省水资源中棘阿米巴属的存在情况。在来自奥尔杜的 17 份天然泉水样本和来自吉雷松的 18 份天然泉水样本中发现了棘阿米巴属。在所调查的任何自来水样本中均未检测到棘阿米巴属。（SSU）rDNA 基因测序结果鉴定出 I 型单倍型（棘阿米巴基因型：KJ094684）。在奥尔杜和吉雷松省，T4（8.6%）是唯一分离出的基因型。这是首次在黑海天然泉水资源中报告棘阿米巴 T4 基因型。棘阿米巴属在天然泉水源中的存在应被视为人类感染的潜在风险，尤其是对高风险人群。
