设计一种新型基于 DNA 的电化学生物传感器，用于选择性和灵敏地测定钡离子。
Designing a novel DNA-based electrochemical biosensor to determine of Ba ions both selectively and sensitively.
机构信息
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
Electroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
出版信息
Anal Biochem. 2022 Apr 1;642:114563. doi: 10.1016/j.ab.2022.114563. Epub 2022 Jan 29.
This work describes a novel electrochemical biosensor based on G-quadruplex (G4) DNA for the sensitive and selective detection of Ba ions using a Carbon Paste Electrode modified with Ag nanoparticles incorporated in reduced Graphene Oxide via a simple wet chemical method (Ag-rGO/CPE), in the presence of carmoisine (CA) as a new electrochemical indicator. The peak current of CA increased with increasing Ba ions concentration and the DNA-based sensor showed linear ranges of 0.06-0.80 nM and 1.0-80 nM and a Limit of Detection (LOD) of 0.045 nM. The proposed biosensor showed a good selectivity toward Ba ions in the presence of other metal ions such as Ag, Hg, K and Na and was applied to the analysis of natural samples showing appropriate results.
这项工作描述了一种基于 G-四链体 (G4) DNA 的新型电化学生物传感器，该传感器使用通过简单的湿法化学方法将 Ag 纳米粒子掺入还原氧化石墨烯中制备的 Carbon Paste Electrode（Ag-rGO/CPE），对 Ba 离子进行灵敏和选择性检测，同时使用胭脂红 (CA) 作为新的电化学指示剂。CA 的峰电流随着 Ba 离子浓度的增加而增加，基于 DNA 的传感器显示出 0.06-0.80 nM 和 1.0-80 nM 的线性范围和 0.045 nM 的检测限 (LOD)。该生物传感器在存在其他金属离子（如 Ag、Hg、K 和 Na）的情况下对 Ba 离子表现出良好的选择性，并应用于天然样品的分析，结果令人满意。
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