MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Biosens Bioelectron. 2022 May 1;203:114030. doi: 10.1016/j.bios.2022.114030. Epub 2022 Jan 25.
Here, an ultrasensitive and highly selective electrochemical biosensor is engineered by integrating bacteria-initiated click chemistry with in situ growth of electroactive polymers. Leveraging the unique copper-binding redox pathway of bacteria to reduce Cu to Cu, Cu-catalyzed click chemistry is initiated and high-density electroactive ferrocenyl polymers are subsequently generated and efficiently grafted on biosensing interface by potentiostatic electrochemical atom transfer radical polymerization that greatly enhances the sensitivity of electro-analytical performance. A good linearity between electrochemical signal and the logarithm of Staphylococcus aureus and Escherichia coli concentration over the range from 10 to 10 CFU/mL is obtained with detection limits down to 4 and 6 CFU/mL, respectively. In order to further expand the applicability and universality of the sensor, bacterial magnetic separation section is supplemented into the system. With the help of aptamer-based magnetic preseparation section, selective detection of target bacteria with great anti-interference is achieved in complex real samples. Moreover, this biosensor can be applied in convenient antibiotics residue detection and rapid drug resistance analysis by merely substitution of recognition element or preincubation of bacteria with different anti-bacteria drugs. Thus, after further expansion of bacterial magnetic separation section or simple replacement of the originally identification element, a universal biosensor including bacteria analysis system and antibiotics detection system with excellent analytical performance is constructed. It provides new insight into the aspects of bacteria-related hazards detection that could not only reduce the detriment caused by bacterial contamination, but also guide antibiotic rational usage and help to control the emergence of multidrug-resistant bacteria.
在这里，通过整合细菌引发的点击化学和电活性聚合物的原位生长，设计了一种超灵敏和高度选择性的电化学生物传感器。利用细菌独特的铜结合氧化还原途径将 Cu 还原为 Cu，引发 Cu 催化的点击化学，并随后通过恒电位电化学原子转移自由基聚合生成高密度的电活性二茂铁聚合物，并有效地接枝到生物传感界面上，从而大大提高了电分析性能的灵敏度。电化学信号与金黄色葡萄球菌和大肠杆菌浓度的对数之间呈现出良好的线性关系，其线性范围为 10 到 10 CFU/mL，检测限分别低至 4 和 6 CFU/mL。为了进一步扩展传感器的适用性和通用性，在系统中补充了细菌磁分离部分。借助基于适配体的磁性预分离部分，在复杂的实际样品中实现了对目标细菌的高选择性和抗干扰检测。此外，通过仅替换识别元件或用不同的抗菌药物预孵育细菌，该生物传感器可用于方便地检测抗生素残留和快速分析耐药性。因此，通过进一步扩展细菌磁分离部分或简单替换最初的识别元件，可以构建一种包括细菌分析系统和抗生素检测系统的通用生物传感器，具有优异的分析性能。它为细菌相关危害检测提供了新的思路，不仅可以减少细菌污染造成的危害，还可以指导抗生素的合理使用，并有助于控制多药耐药菌的出现。
